分子束中生物分子的光谱与结构  

Spectra and Structures of Biomolecules in the Molecular Beam

作　　者：胡勇军[1] 邢达[1] 

机构地区：[1]华南师范大学生物光子学研究院激光生命科学研究所暨激光生命科学教育部重点实验室,广州510631

出　　处：《化学进展》2009年第7期1592-1602,共11页Progress in Chemistry

基　　金：广东省自然科学基金项目(No.07005823);华南师范大学激光生命科学教育部重点实验室开放课题基金资助

摘　　要：研究分子束中的生物分子有助于探讨生物分子的固有性质和由于生物环境而引起的其他性质之间的区别。本文首先介绍了一种可将生物分子在气相中"孤立"的实验技术,即激光解吸并分子束冷却技术,同时还介绍了与该研究方向相关的光谱方法,即UV/UV和IR/UV烧孔光谱等。并回顾了近年来人们应用这些实验技术对分子束中的氨基酸和短肽、核酸碱基、糖类和神经递质类分子以及它们的团簇最新研究进展。文中还简要介绍了一种新的应用于构象异构动力学研究的光谱方法,即激励辐射泵浦-注孔(SEP-HF)和激励辐射泵浦-布居转移(SEP-PT)。最后,综述了短肽离子光解离动力学和短肽离子中发生的电荷快速转移动力学过程的研究进展。Study of the biomolecules in the molecular beam is a good molecular properties and properties resulting from the biological environment. isolate the biomolecules in the gas phase, i.e. laser desortpion/jet cooling, approach to distinguish between intrinsic In this review, an experimental approach to has been introduced at first. The related spectroscopic techniques, i.e. UV/UV spectroscopy and IR/UV hole burning （HB） spectroscopy, have been presented. Thereafter, the recent progress in the study of amino acids and peptides, DNA bases, sugars, neurotransmitters molecules and their clusters in the gas phase has been reviewed. Thirdly, two novel spectroscopic techniques, SEP-PT （stimulated emission pumping-population transfer）and SEP-HF （stimulated emission pumping-hole filling）, have been introduced. By employ those techniques, the energy thresholds for conformational isomerization in a given reactantproduct conformer pair （e. g. tryptamine） are directly determined. In the final part of the article, the studies on the fast charge transfer dynamics in the peptide ions have been reviewed.

关 键 词：生物分子 光谱 分子束 分子动力学 

分 类 号：O657.3[理学—分析化学] O641.3[理学—化学]