急性胰腺炎患者Toll样受体4基因多态性分析研究  

Toll like receptor 4 polymorphism in patients with acute pancreatitis

作　　者：陈文亮[1] 李文龙[1] 苏继荣[1] 韩曙光[1] 义晓燕[1] 

机构地区：[1]山西医科大学第二医院普外科,太原030001

出　　处：《中国药物与临床》2009年第8期685-688,共4页Chinese Remedies & Clinics

基　　金：山西省卫生厅科技攻关项目(200408)

摘　　要：目的研究Toll样受体4(TLR4)突变与急性胰腺炎(AP)发展及预后的相关性。方法采用错配聚合酶链反应(PCR)-限制性片段长度多态性分析方法在AP和健康志愿者人群中检测TLR4(896A>G)的突变频率。结果①所有TLR4的突变均为杂合子;②AP组TLR4(-/+)基因型频率在轻症AP组和重症AP中呈轻度升高,与健康志愿者组比较差异无统计学意义(P>0.05);③在AP组中,TLR4(-/+)基因型频率呈波浪形升高趋势,并在胰腺组织坏死合并感染组达到峰值;与健康志愿者组相比,胰腺组织坏死合并感染组TLR4(-/+)等位基因型频率差异有统计学意义(P<0.05);④与无胰腺组织坏死组相比,胰腺组织坏死组TLR4(-/+)基因型频率明显升高,差异有统计学意义(P<0.05)。结论TLR4错义突变(TLR4 896A>G)在AP发生发展中具有重要的作用,TLR4的基因突变可以损害机体的防御反应。Objective To determine the relationship between the toll-like receptor 4 （TLR4） mutations and development and progression of acute pancreatitis （AP）. Methods TLR4 896A〉G mutation was surveyed by mismatch PCR-restriction fragment-length polymorphism analysis technique in AP patients and healthy volunteers. Results ①All individuals with Toll like receptor 4 mutations were genetically heterozygous; ②The allele frequency of the TLR 4 （896A〉G） was 5.7% among 53 healthy volunteers, 10% among mild acute pancreatitis patients and 9.9% among severe acute pancreatitis （SAP） patients;③ Compared with healthy volunteers, there was a significant trend towards rising frequency of TLR4（896A〉G） mutations in AP patients with pancreatic necrosis and secondary infections （P〈0.05） ;④The allele frequency of TLR 4 （896A〉G） increased significantly in SAP patients with pancreatic necrosis as compared to those without necrosis. Conclusion Human TLR4 （896A〉G） mutations may be playing an important role in the pathogenesis of AP by harmful effects on defense response of the body.

关 键 词：TOLL样受体4 多态性 单核苷酸 胰腺炎 错配PCR-限制性片断长度多态性分析 

分 类 号：R576[医药卫生—消化系统]