余甘子叶化学成分没食子酸对人肝癌BEL-7404细胞株凋亡的影响  

The Effect of Gallic Acid Extracted from Leaves of Phyllanthus emblica on Apoptosis of Human Hepatocellular Carcinoma BEL-7404 Cells

作　　者：钟振国[1] 黄金兰[1] 梁红 钟益宁[1] 张雯艳[1] 吴登攀[1] 曾春兰[3] 王进声[1] 韦勇汉[1] 

机构地区：[1]广西中医学院,广西南宁530001 [2]广西药科学校,广西南宁530023 [3]广西工业职业技术学院食品与生物工程系,广西南宁530001

出　　处：《中药材》2009年第7期1097-1101,共5页Journal of Chinese Medicinal Materials

基　　金：广西科学技术厅项目(桂科基0639051;桂科攻0718002-2-1);广西高校人才小高地建设创新团队资助计划;广西研究生教育创新计划资助项目(桂中研「2008」24号)

摘　　要：目的:探讨余甘子叶化学成分没食子酸对人肝癌细胞株BEL-7404凋亡的影响。方法:采用MTT法测定余甘子叶化学成分没食子酸在体外对BEL-7404细胞增殖的影响;普通光学显微镜、倒置相差显微镜和荧光显微镜观察余甘子叶化学成分没食子酸作用BEL-7404细胞后的形态学变化;AnnexinV/PI双标记法流式细胞术检测余甘子叶化学成分没食子酸诱导BEL-7404细胞早期细胞凋亡的情况;Tunel法检测BEL-7404细胞的晚期凋亡情况及其凋亡率。结果:MTT法和显微镜观察显示余甘子叶化学成分没食子酸能不同程度抑制BEL-7404细胞的增殖,可致细胞形态学改变,出现典型的凋亡特征;AnnexinV/PI双标记法检测显示早期凋亡细胞随作用时间的延长而增加,Tunel法检测晚期凋亡细胞,其凋亡率亦随作用时间的延长而增大。结论:余甘子叶化学成分没食子酸能抑制BEL-7404细胞的增殖并诱导其产生凋亡,其对BEL-7404细胞的凋亡影响呈时间依赖性。Objective： To explore the effect of gallic acid extracted from Leaves of Phyllanthus emblica on the apoptosis of BEL- 7404 cells. Methods： MTT assay was applied to detect the influence on proliferation in vitro. Inverted microscope was utilized to observe the morphological changes after BEL-7404 cells were treated with gallic acid. Annexin V/PI double label method was used to detect earlier period apoptosis cells and Tunel was applied to calculate the apoptosis rates. Results： Gallic acid could restrain the BEL- 7404 ceils proliferation at diffierent levels in a time and concentration dependent manner. The typical morphological changes of apoptosis were observed after BEL-740d cells were treated with gallic acid. Annexin V/PI double label method and Tunel method showed that the viable apoptotic cell and apoptosis rates added as action time prolonged. Conclusion： Gallic acid can restrain the BEL-7404 cells proliferation and induce apoptosis, and its effect on apoptosis is time dependent.

关 键 词：余甘子叶 没食子酸 BEL-7404细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]