纳米硒对性成熟前雄性波尔山羊生殖机能发育的影响  

Effect of Nanometer-Selenium on the Development of Reproductive Function in Prepuberital Male Boer Goat

作　　者：杨茹洁[1] 施力光[1] 岳文斌[1] 荀文娟[1] 张春香[1] 任有蛇[1] 赵平伟 雷晓峰[3] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]山西省兽药饲料监察所,太原030027 [3]山西农业大学试验基地黎城县种羊场,山西黎城047600

出　　处：《中国农业科学》2009年第8期2923-2929,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30371045)

摘　　要：【目的】研究日粮添加纳米硒对断奶雄性山羊性成熟过程中生殖机能的影响。【方法】选用2月龄断奶、体重一致的波尔山羊公羔羊20只,随机分成2组,对照组饲喂基础日粮,处理组饲喂基础日粮+纳米硒(硒含量0.3mg·kg-1),预饲10d,试验期90d,每30d采血分离血清测定FSH、LH和T浓度,分离睾丸测定睾丸硒浓度和GSH-Px活性,同时制作常规石蜡切片观察睾丸组织结构。试验结束时采集精液分析精液品质,分离精子,透射电镜观察精子超微结构。【结果】纳米硒可促进睾丸曲精细管和间质组织及间质细胞发育,维持曲精细管中生殖细胞的发生、分化及分裂;日粮补充纳米硒极显著增加睾丸硒沉积、提高睾丸GSH-Px活性(P<0.01);30～60d阶段纳米硒不影响血清生殖激素水平(P>0.05),0～90d阶段FSH、LH和T显著升高;纳米硒对射精量和精子密度无影响,精子活力增加10%,畸形精子数目降低27%。透射电镜观察精子超微结构,对照组精子尾部中段线粒体结构改变,排列疏松。【结论】纳米硒显著影响性成熟前公羔羊睾丸的发育,增加精子活力,降低畸形精子数,对血清生殖激素水平有一定影响。[ Objective ] The experiment was conducted to study the effect of nano-selenium on reproductive function in male Boer goat from weaned to sexual maturity. [Method] Total 20 healthy goats were randomly divided into two treatments in 10 replications, and they were fed with a basal diet supplemented with 0 （control） or 0.3 mg,kgl Se from nano-selenium. The experiment lasted for 90 d before 10 weeks pretesting, and the blood samples were collected and separated serum for sexual hormones every 30 days. The testis obtained, measured the Se concentration and the activity of GSH-Px, fixed and made the microscopic section, observed the testicular microstructure. At the end of experiment, semen quality in each group was analyzed, ultrastructure of spermatozoa was observed through TEM. [Result] The results showed that the development of seminiferous tubules, interstitial tissue and germ cells were affected by supplementing nano-Se in basal diets. Dietary supplementation with nano-Se increased testis Se content, the activity of GSH-Px （P〈0.01） significantly. Testosterone, luteinizing hormone and follitropin were not affected by supplementation Se during the phase of 30-60 d, and increased significantly in the phase of 90 d compared with control. Sperm activity increased by 10% and abnormality sperm decreased by 27%, respectively （P〈0.05）, and no change in sperm quantity and density was observed. The longitudinal sections of midpiece of sperm mitochondria showed the change of the biological characters and arranged loosely. [ Conclusion] These results indicated that supplementation of nano-Se affected the reproductive function ofprepubertital goat, and improved the sperm quantity.

关 键 词：纳米硒 断奶公羔 生殖激素 睾丸曲精细管 生殖细胞 超微结构 

分 类 号：S827[农业科学—畜牧学] X503.1[农业科学—畜牧兽医]