青钱柳叶中黄酮类化合物的地理变异  

Geographic variation of flavonoid compounds in Cyclocarya paliurus leaves

作　　者：杨万霞[1] 佘诚棋[1,2] 方升佐[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]安徽省林业科技推广总站,安徽合肥230001

出　　处：《浙江林学院学报》2009年第4期522-527,共6页Journal of Zhejiang Forestry College

基　　金：江苏省高新技术研究项目(BG2006314)

摘　　要：以青钱柳Cyclocarya paliurus11个种源1年生苗的叶片为材料,研究青钱柳叶片中黄酮类化合物的地理变异,初步筛选出黄酮类化合物质量分数高的优良药用种源。黄酮类化合物各成分用液相色谱测定,数据采用方差分析、多重比较和相关分析等进行统计分析。结果表明:青钱柳叶中3种黄酮类化合物质量分数紧密相关,其中山奈酚质量分数高于槲皮素和异槲皮苷。研究的各种源中,安徽歙县和广西资源种源的黄酮类化合物的质量分数较高,分别为6.770和6.494mg·g-1,安徽舒城种源的黄酮类化合物质量分数最低,仅为1.230mg·g-1;以经纬度为自变量,各成分的质量分数为因变量进行趋势面分析,青钱柳叶中山奈酚质量分数呈经纬度双向变异,但其变化幅度随经度变化幅度不如纬度变化大,其表现出以108°E和27°N交叉区域为中心向东西递增、向南北递减的趋势,而槲皮素和异槲皮苷质量分数的变化没有明显的规律性。青钱柳黄酮类化合物较高的优良药用种源为安徽歙县和广西资源种源。To select superior medicinal provenances of Cyclocarya paliurus, the geographic variation of flavonoid compounds （kaempferol, quercetin, and isoquercetin） in leaves of one-year-old seedlings of 11 provenances was studied. Flavonoid compounds were measured by HPLC. Statistical analyses, such as variance analysis, correlation analysis were used. Results showed a close relationship existed among the three flavonoid compounds with kaempferol content greater than quercetin and isoquercetin. Flavonoid content in Shexian provenance of Anhui （6.770 mg.g^-1） and Ziyuan provenance （6.494 mg. g^-1） of Guangxi were higher, whereas Shucheng provenance of Anhui was lowest （1.230 mg. g^-1）. Trend surface analysis of geographic variations was made with longitude and latitude being independent variable, the content of flavonoids compounds being induced variable, The kaempferrol showed a center （crossing of 108°E and 27° N）-round variation, with the increase from the center to the west and east , decrease from the center to the north and south. However, there was no significant trend in the variation of quercetin and isoquercetin. The superior medicinal provenances of Cyclocarya paliurus is Shexian provenance of Anhui and Ziyuan provenance of Guangxi. [Ch, 4 fig. 3 tab. 15 ref.]

关 键 词：林木育种学 青钱柳 山奈酚 槲皮素 异槲皮苷 地理变异 

分 类 号：S722.5[农业科学—林木遗传育种]