肺栓塞患者免疫防御系统相关基因mRNA表达及意义  

The expression and significance of immunity associated genes mRNA in patients with pulmonary embolism

作　　者：龚朱[1] 梁爱斌[2] 王乐民[1] 张晓宇[1] 王嫱[1] 黄春瑜[1] 宋浩明[1] 汪浩[1] 沈玉芹[1] 郜恒骏[3] 沈晓荧[3] 

机构地区：[1]同济大学附属同济医院心内科,上海200065 [2]同济大学附属同济医院血液科,上海200065 [3]生物芯片上海国家工程研究中心

出　　处：《中华内科杂志》2009年第8期666-669,共4页Chinese Journal of Internal Medicine

基　　金：国家自然科学基金（30570809;30871080）

摘　　要：目的探讨肺栓塞（PE）患者免疫功能状态在分子水平的变化。方法采用全人类基因组表达谱芯片检测PE组和对照组免疫系统相关基因表达，运用随机方差模型校正的参数t检验进行2组标准化基因强度数据比较。结果与对照组比较，PE组中性粒细胞、单核巨噬细胞相关基因、干扰素调控因子、TNF基因、NK细胞功能相关基因、黏附分子相关基因、T细胞功能基因表达差异有统计学意义，而补体途径激活因子基因、B细胞功能相关基因表达差异无统计学意义。结论PE患者免疫系统功能呈现失衡性表达，T细胞免疫功能相关基因下调提示PE病因学与病毒感染相关。Objective To investigate the molecular alteration of immunity associated genes in patients with pulmonary embolism （PE） so as to preliminarily elucidate its pathogenetie mechanism. Methods Human cDNA microarray analysis was employed in this study, random variance model （RVM） corrected t-test was used for the statistical data analysis of differential gene expression. Results In comparison with control, mRNA expression of functional genes of neutrophils, monophagocytes, IFN regulating factors, TNF, adhesion molecules and T cells were significantly different in PE patients. However, gene expressions of B cell immune function and complement activation associated factors were not significantly different between two groups. Conclusion Unbalance expression of immune function associated genes, especially down-regulated expression of T cell mediated function genes, in patients with PE indicates that the etiology of PE might be related to viral infection.

关 键 词：肺栓塞 免疫系统 基因 失衡 

分 类 号：R686[医药卫生—骨科学]