猪背最长肌中胰岛素样生长因子(IGFs)系统基因的发育表达模式  

Developmental expression changes of insulin-like growth factors(IGFs) system genes in longissimus dorsi muscle of two pig breeds

作　　者：顾以韧[1] 张凯[1] 李明洲[1] 李学伟[1] 朱砺[1] 王金勇 陈磊 

机构地区：[1]四川农业大学动物科技学院,雅安625014 [2]重庆市畜牧科学研究院,荣昌402460

出　　处：《遗传》2009年第8期837-843,共7页Hereditas（Beijing)

基　　金：国家现代农业产业技术体系专项(编号:NYCYTX-009);国家公益性行业(农业)科研专项(编号:NYHYZX07-034);国家自然科学基金(编号:30771534);四川省科技厅"十一五"畜禽育种攻关项目(编号:2006YZGG-15)资助

摘　　要：采用荧光定量PCR技术检测了长白猪和梅山猪的背最长肌组织中胰岛素样生长因子1和2(IGF-1和-2)、胰岛素样生长因子1受体和2受体(IGF-1R和-2R)、胰岛素样生长因子结合蛋白3和5(IGFBP-3和-5)基因 mRNA丰度在初生(0月龄)、1、2、3、4和5月龄间的表达变化并分析品种间和不同月龄间基因表达的差异及其对肌肉生长发育的影响。结果表明:两猪种出生后IGF-1 mRNA表达量均表现为逐渐上调,而IGF-2则恰好相反,表现为逐渐下调。这与IGF-2主要在胚胎期发挥作用,而IGF-1则主要在动物个体出生后才发挥促进细胞增殖和个体发育功能的特点相符。IGFRs mRNA与IGFs mRNA表达的发育性变化模式并不相似,提示背最长肌组织中IGFRs mRNA的表达可能没有受到组织局部产生的IGFs调节。长白猪的IGF-1R、IGF-2R和IGFBP-3 mRNA表达量均在2月龄时达到最高峰,提示2月龄可能是长白猪IGFs系统发挥作用最为明显的生长发育阶段。以上结果初步揭示猪生长发育过程中胰岛素样生长因子系统基因表达的发育性变化模式和品种差异,为深入研究胰岛素样生长因子系统基因的相互调控机制提供了基础数据。Real-time fluorescence quantitative PCR was applied to investigate the developmental expression patterns of insulin like growth factor-1 and -2 （1GF-1 and -2）, insulin like growth factor receptor -1 and -2 （1GF-1R and -2R）, insulin like growth factor binding protein-3 and -5 （1GFBP-3 and -5） genes at the age of birth （0）, 1, 2, 3, 4, and 5 months in longissimus dorsi muscle of Landrace and Meishan pigs. The expression level oflGF-1 exhibited up-regulation, in contrast, the expression level oflGF-2 showed down-regulation after birth in the two pig breeds. These findings are consistent with the previous knowledge that IGF-2 is mainly expressed during the embryonic phase, IGF-1 is mainly expressed during the postnatal period, which could promote cell proliferation and ontogenesis. The expression patterns oflGFRs and IGFs were dissimilar from 0 to 5 months in the two pig breeds, which suggest that the expression changes oflGFRs may not be subjected to the regulation by local secretion of IGFs. Strikingly, at the age of 2 months, the expression levels of IGF-1R, 1GF-2R, and IGFBP-3 reached their maximum peak values in Landrace pigs, which suggest that the 2 months after birth may be the most important growth stage for genes of insulin-like growth factors （IGFs） system to exert their biological functions from birth to 5 months. These results indicate that the mRNAs of IGFs system genes exhibit specific developmental expression changes between Landrace and Meishan pigs, and provides some data for further study on the mutual regulation mechanism of genes in IGFs system.

关 键 词：猪 背最长肌 荧光实时定量PCR 胰岛素样生长因子 胰岛素样生长因子受体 胰岛素样生长因子结合蛋白 

分 类 号：S828[农业科学—畜牧学]