透明质酸寡糖调节A549/DDP多药耐药作用的研究  

Effects of reversing drug resistant of hyaluronate oligomers on A549/DDP cell line of human lung cancer

作　　者：张宪真[1] 王宝中[1] 

机构地区：[1]山东聊城市人民医院肿瘤科,山东聊城252000

出　　处：《现代肿瘤医学》2009年第8期1412-1414,共3页Journal of Modern Oncology

摘　　要：目的:通过研究透明质酸寡糖对人肺腺癌细胞系A549/DDP的P糖蛋白和多药耐药相关蛋白(MRP)、肺耐药蛋白(LRP)表达的影响,探讨透明质酸在引起肿瘤细胞多药耐药中的作用。方法:将CD44单抗或透明质酸寡糖与A549/DDP细胞共培养48小时,放免法检测培养基中细胞所分泌透明质酸的含量,流式细胞仪检测经上述处理的A549/DDP表面MDR1、MRP、LRP的表达率,MTT法检测在不同浓度顺铂作用下,各组细胞的存活率。结果:经透明质酸寡糖处理后A549/DDP,分泌透明质酸较未处理组明显减少(P<0.05);且细胞表面与多药耐药相关的MDR1、MRP、LRP的表达率均降低(P<0.05)。另外,处理后的细胞,在不同浓度顺铂作用时,细胞凋亡率明显增加。结论:体外条件下,透明质酸寡糖能逆转A549/DDP的耐药。Objective：To investigate the effects of hyaluronate oligomers on the multiple drug resistance of lung cancer cell lines A549/DDP. Methods ： After co - culturing A549/DDP and CD44 monoclonal antibody or hyaluronan oligomers for d8 hours, to detect the following parameters ： Hyaluronate contents of the medium by radioimmunoassay, MDRI, MRP, LRP expressions by flow cytometry, survival rate of ceils under different concentrations of cisplatin by MTT tests. Results： Hyaluronan oligomers can decrease hyaluronan expression, and MDR1, MRP, LRP expression of A549/DDP. In addition, apoptosis level of cells treated by hyaluronan oligomers increased significantly in higher concentration cisplatin. Conclution： In vitro,hyaluronan oligomers can reverse drug resistance of A549/DDP.

关 键 词：A549 CD44单抗 透明质酸寡糖 P糖蛋白 

分 类 号：R730.2[医药卫生—肿瘤]