奥曲肽对人胃癌BGC-823细胞增殖和凋亡影响的研究  

Effect of octreotide on cells proliferation and apoptosis in human gastric cancer cell line BGC-823

作　　者：孙海英[1,2] 张琍[1] 李国庆[1] 刘红英[1] 陈方志[1] 

机构地区：[1]南华大学附属第二医院消化内科,湖南衡阳421001 [2]青岛市传染病医院,山东青岛266000

出　　处：《现代肿瘤医学》2009年第8期1426-1430,共5页Journal of Modern Oncology

基　　金：南华大学附属第二医院科研课题(Y2006-41)

摘　　要：目的:研究生长抑素类似物奥曲肽(octreotide,OCT)对胃癌BGC-823细胞增殖、凋亡、细胞周期的影响及其可能的作用机制。方法:不同浓度的OCT作用于体外培养的人胃癌BGC-823细胞,以5-FU作为阳性对照,应用MTT法分析细胞增殖的抑制作用,流式细胞仪测定细胞周期分布和凋亡率,AO-EB染色观察细胞形态变化。结果:OCT对BGC-823细胞的生长、增殖产生抑制作用,该抑制作用具有量效、时效关系和饱和性;OCT作用后细胞呈典型的凋亡形态学改变,流式细胞仪检测的细胞凋亡率和AO-EB染色测得细胞凋亡率与对照组相比较差异有显著性(P<0.05);BGC-823细胞经OCT作用后,G1期细胞数明显增加,S细胞数明显减少,细胞被阻滞于G1/S期。结论:10-5-10-3g/L浓度的OCT能够抑制胃癌BGC-823细胞增殖,机制可能与其诱导肿瘤细胞的凋亡和出现G1/S期阻滞有关。Objective：To study the effect of octreotide（OCT） on proliferation,apoptosis and cell cycle in human gastric cancer cell line BGC - 823 in vitro, and further to explore the possible mechanism of inhibition. Methods： Human gastric cancer cell line BGC - 823 was treated with Octreotide. 5 - FU was used as positive control. MTT assay and AO - EB fluorescent staining as well as flow cytometry were performed in this study. Results： Octreotide inhibited the growth and proliferation of BGC - 823 in vitro within certain concentrations. The suppression was dose - dependent, time - dependent, and had staturability. Morphological variations of apoptosis were observed after treated with OCT, the apoptotic rate measured by AO -EB fluorescent staining and flow cytometry had statistical significance compared with negative group（ P 〈 005 ）. G1 phase rate gradually increased, S phase rate gradually decreased, cell cycle was arrested at Glphase in human gastric cancer cell line BGC -823 treated by OCT. Conclusion： Octreotide can inhibit the proliferation of human gastric cancer cell line BGC - 823 in vitro. It might be related with inducing apoptosis and arresting cell cycle at G1/S phase.

关 键 词：胃癌 奥曲肽 BGC-823 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]