银杏叶提取物（EGB）对局灶性脑缺血大鼠脑片HIF-1 VEGF表达及新生血管的影响  

Expression of VEGF and HIF - 1 after focal cerebral ischemia reperfusion in kats

作　　者：梅松柳[1] 高俊霞[1] 杨立杰[1] 

机构地区：[1]牡丹江医学院红旗医院急诊科,黑龙江157000

出　　处：《中国急救医学》2009年第8期724-726,F0003,共4页Chinese Journal of Critical Care Medicine

基　　金：黑龙江省卫生厅课题（No.2007-057）

摘　　要：目的探讨银杏叶提取物（EGB）对大鼠局灶性脑缺血／再灌注中脑组织内血管内皮细胞生长因子（VEGF）及血管通透因子（HIF-1）表达的影响。方法采用Zea Longa线栓法制备大鼠局灶性脑缺血／再灌注模型（即MCAO模型），54只大鼠随机分为三组，即对照组（n=6）、EGB组（n=24）及缺血／再灌注组（n=24），分别在缺血／再灌注4、6、24、72h取材。应用免疫组化法（SABC法），利用计算机图像分析系统软件，计数脑梗死后不同时间点阳性细胞的数量。检测VEGF及HIF—1蛋白的表达变化情况。结果对照组：脑组织VEGF和HIF表达均较低；EGB组：VEGF及HIF阳性细胞密度均相对较高；缺血／再灌注组：VEGF在4h开始升高，24h达到高峰，72h下降到接近正常水平，HIF-1在缺血后4h开始降低，8h开始升高，24h达到高峰，72h下降到接近正常水平。结论EGB对于缺血性脑损伤有保护作用。Objective To observe the effect of extract of Ginkgo biloba （ EGB ） on the expression of vascular endothelial growth factor （ VEGF ） and HIF - 1 after focal cerebral ischemia reperfusion in rates. Methods MCAO （ Middle Cerebral Artery Occlusion ） models were set up in rats. 54 male Wistar rates were randomized into three groups ： control group, EGB groups and groups of isehemia for 2 h then respective reperfusion for 4 h, 6 h, 24 h, 72 h. tmmunohistochemistry （SABC）were used to detect the VEGF and HIF - 1 protein expression. Results VEGF and HIF - 1 protein expression in control group were remarkably deep, and it was strongest in EGB groups. VEGF increased at 4 h, reached peak at 24 h, then returned to normal level at 72 h, while HIF - 1 increased at 8 h , reached peak at 24 h, then returned to normal level at 72 h. Conclusion EGB has neuroprotective effect against cerebral ischemie injury.

关 键 词：银杏叶提取物 局灶性脑缺血 血管通透因子 血管生长因子 

分 类 号：R743.31[医药卫生—神经病学与精神病学]