新疆核桃品种试管苗不定根发生的影响因素研究  

Factors Affecting in vitro Rooting of Xinjiang Walnut Cultivars

作　　者：胡石开[1,2] 王晓军[1] 郝秀英[3] 唐晓义[1,2] 李静[1,2] 牛力涛[1,2] 努尔.波拉提[1,2] 

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [2]中国科学院研究生院,北京100039 [3]新疆农业科学院微生物研究所,新疆乌鲁木齐830091

出　　处：《北方园艺》2009年第8期107-109,共3页Northern Horticulture

基　　金：中国科学院西部行动高新技术资助项目(KGCX2-SW-506)

摘　　要：以新疆2个优良核桃品种新温185号、新温2号为试材,对影响试管苗的不定根发生的影响因素进行了研究。结果表明:基本培养基、外源IBA水平、光周期及黑暗条件对不定根发生具有一定的影响。选取生长旺盛的试管苗,采用一步浸蘸诱导生根法,即将试管苗放入经常规灭菌处理过的80 mg/L的IBA溶液中浸蘸处理75 min,然后转入不含任何激素的1/4 DKW中,黑暗处理12 d后置于光照下生根。利用这一方法快速诱导了试管苗生根,获得了高达90.90%的生根率,生根指数达254.44。The factors affecting in vitro rooting of Xinjiang walnut cultivars-Xinwen 185 and Xinwen 2 were researched. The results showed that the rooting was related to the basic medium, the level of foreign IBA, light and dark conditions. The rooting rate of 90. 90% and root index of 254. 44 were achieved in two cultivars by using a step-induced root dip method that was firstly induced in 80 mg/L of IBA solution for 75 minutes, and then transferred to the 1/4DKW medium which did not contain any hormone,and then took to induce rooting under the light after in dark condition for 12 days.

关 键 词：核桃品种 生根 离体培养 新疆 

分 类 号：S664.103.6[农业科学—果树学]