莲藕腐败病病原菌人工接种致病性测定方法的研究  

Determination Method for Pathogenicity of Artificial Inoculated Fusarium Pathogen of Lotus

作　　者：魏林[1,2] 曹福祥[1] 梁志怀 安哲宇[3] 

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]湖南省植物保护研究所,湖南长沙410125 [3]中南大学研究生院隆平分院,湖南长沙410125

出　　处：《湖南农业科学》2009年第7期74-76,共3页Hunan Agricultural Sciences

基　　金：湖南省博士后科研资助专项计划项目(2008RS4028);国家"十一五"科技支撑计划项目(2006BAD17B08)

摘　　要：为确定室内筛选抗腐败病(Fusarium)莲藕品种及测定该病病原菌致病性分化的适宜方法,对莲藕腐败病病原菌进行了菌丝体牙签接种、菌丝块接种和孢子悬浮液喷雾接种等人工接种方法的致病性测定。实验结果表明,用病原菌菌丝块对嫩藕藕块进行接种发病率最高,发病最重,可作为室内莲藕抗腐败病品种筛选、病原菌致病性室测定的首选接种方法。此外,试验结果还表明,来源于非莲藕寄主的镰刀菌对莲藕具有一定的侵染性,使莲藕呈现典型的腐败病症状。In order to select the fusarium-resistant variety of lotus and find out the method for determining the pathogenicity of fusarium pathogen, four artificial inoculation experiments for pathogenicity determination of fusarium pathogen were conducted, which using lotus root with mycelium block, lotus root with mycelium, lotus leaf with mycelium block and lotus leaf with spore suspension spray respectively. The results showed that pathogen mycelium block inoculation caused the highest disease rate and disease index. It could use to select the fusarium-resistant variety of lotus and determine the pathogenicity of fusarium pathogen. The results also indicated that the fusarium from the non-lotus host has certain infectivity to the lotus, which will appear the typical symptom of rot.

关 键 词：莲藕 腐败病菌 人工接种 致病性 

分 类 号：S436.45[农业科学—农业昆虫与害虫防治]