塞来昔布对Tca8113细胞环氧化酶-2表达及诱导凋亡的作用  

Effect of Celecoxib on cycloxygenase-2 expression and inducing apoptosis in Tca8113 cell lines

作　　者：李伟忠[1] 王晓燕[2] 丁彦青[2] 

机构地区：[1]南方医科大学南方医院口腔科,广东广州510515 [2]南方医科大学南方医院病理科,广东广州510515

出　　处：《华西口腔医学杂志》2009年第4期374-377,385,共5页West China Journal of Stomatology

基　　金：广东省自然科学基金资助项目(06024396)

摘　　要：目的观察选择性环氧化酶-2(COX-2)抑制剂塞来昔布对人舌鳞癌Tca8113细胞的生长抑制、COX-2表达调节及诱导凋亡的作用。方法在Tca8113细胞中分别加入含有不同浓度塞来昔布的培养液,培养24、48、72h后,采用MTT方法测定细胞生长抑制率,采用免疫组化方法观察COX-2蛋白在Tca8113细胞中的表达,应用荧光显微镜观察凋亡细胞的形态学特征,应用Annexin V-FITC/PI双标记法检测细胞的早期凋亡率,相对定量荧光定量PCR检测Tca8113细胞中COX-2 mRNA的表达。结果COX-2蛋白在Tca8113细胞中呈强阳性表达,塞来昔布在抑制细胞生长的同时,抑制Tca8113细胞COX-2蛋白的表达;Tca8113细胞经塞来昔布处理后凋亡细胞多见,与对照组相比,塞来昔布处理组早期凋亡细胞增多有统计学意义;塞来昔布调节COX-2 mRNA表达的作用与对照组相比无统计学意义。结论塞来昔布主要以剂量依赖的方式抑制Tca8113细胞生长,诱导细胞凋亡,其作用与抑制COX-2蛋白表达有关,而对COX-2基因作用较弱,其作用机制有待进一步研究。Objective To observe whether Celecoxib could inhibit the growth, regulate the expression of COX-2 and induce apoptosis of Tca8113 cells. Methods Tca8113 cells were incubated with different concentrations of Celecoxib for 24, 48 and 72 h, and MTT was used to calculate growth inhibition rate. The expression of COX-2 protein and mRNA in Tca8113 cells was detected with SP immunohistochemistry staining and fluorescent quantitative realtime RT-PCR. Morphology of apoptosis cells was observed by fluorescence microscopy, and Annexin V-FITC/PI double labeling method was employed to detect early stage cell apoptosis. Results COX-2 protein was strongly expressed in Tca8113 cells and was suppressed by Celecoxib. The growth and proliferation of Tca8113 cells treated with Celecoxib were inhibited in a dose-dependent manner. Celecoxib treatment resulted in significant increase in apoptosis and early apoptotic rate. Fluorescent quantitative real-time RT-PCR results showed no significant effet on regulating expression of COX-2 mRNA. Conclusion Celecoxib shows a significant effect on inhibiting expression of COX-2 in Tca8113 ceils, this is probably related to growth inhibition and inducing apoptosis of Tca8113 cells.

关 键 词：塞来昔布 环氧化酶-2 细胞凋亡 鳞状细胞癌 

分 类 号：R739.8[医药卫生—肿瘤]