促性腺激素与IL-6对上皮性卵巢癌细胞增殖影响的研究  

Effects of Gonadotropins and IL-6 on Proliferation in Epithelial Ovarian Carcinoma Cells

作　　者：杜湧瑞[1,2] 陈颖[2] 高燕[2] 邓为民[1] 姚智[1] 

机构地区：[1]天津医科大学免疫教研室,300070 [2]天津市中心妇产科医院

出　　处：《天津医药》2009年第8期625-627,I0001,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30670801);天津市应用基础研究计划面上项目(项目编号:06YFJMJC08300)

摘　　要：目的:探讨促性腺激素及白细胞介素-6(IL-6)在卵巢癌细胞增殖中的作用。方法:将不同剂量的人重组促性腺激素、IL-6与人卵巢低分化乳头状腺癌细胞株(OVCAR-3)共培养,应用四甲基偶氮唑蓝(MTT)实验观测其对卵巢癌细胞生长的影响。IL-6的表达分别采用RT-PCR和ELISA法进行检测,IL-6受体(IL-6RΑα和gp130)的表达采用RT-PCR及免疫印迹技术进行测定。结果:促性腺激素(20、60、100U/L)、IL-6(20、40、60、80、100μg/L)对OVCAR-3具有促增殖作用,与对照组相比差异有统计学意义,同时细胞培养上清液中IL-6含量、细胞IL-6受体mRNA及蛋白表达水平有增高趋势。结论:促性腺激素对上皮性卵巢癌细胞株的促增殖过程可能由IL-6介导,促性腺激素、IL-6共同影响上皮性卵巢癌细胞的生长。Objective： To investigate the effects of gonadotropins and interleukin-6（IL-6） on the proliferation in epithelial ovarian carcinoma cells （OVCAR-3） . Methods：Epithelial ovarian carcinoma cell line was co-cultured with follicle- stimulating hormone （FSH）, luteinizing hormone （LH） or IL-6. MTT was performed to examine the proliferation of the ovarian carcinoma cells. Reverse transcription polymerase chain reaction （RT-PCR） and enzyme linked immunoabsorbent assay （ELISA） were performed to examine the expression of IL-6. The expression of IL-6 receptor （IL-6Rα, gp130） was detected by Western blot analysis. Results：Dose-response effects of gonadotropins（20, 60 and 100 U/L） and IL-6 （ 20, 40 60, 80 and 100 μg/L） were seen on proliferation of OVCAR-3. There was an increased tendency in the expressions of IL-6 receptor mRNA in cell culture supernate. Conclusion： The results suggest gonadotropins-stimulated cell proliferation is mediated by IL-6. Gonadotropins and IL-6 are possibly responsible for the prognosis of the ovarian cancer.

关 键 词：卵巢肿瘤 卵泡刺激素 促黄体激素 白细胞介素-6 细胞增殖 

分 类 号：R737.31[医药卫生—肿瘤] R733.7[医药卫生—临床医学]