激光拉曼光谱在胃癌研究中的应用  

Application of laser resonance Raman spectroscopy in gastric cancer

作　　者：冷爱民[1] 王华秀[1] 阳静[1] 崔建芳[1] 张桂英[1] 

机构地区：[1]中南大学湘雅医院消化科,湖南长沙410008

出　　处：《中国现代医学杂志》2009年第13期2015-2019,共5页China Journal of Modern Medicine

摘　　要：目的研究激光拉曼光谱技术应用于胃癌研究的可能性。方法收集胃癌患者手术前、术后3d的血清及健康人血清和SGC7901胃癌细胞培养24h、48h和72h的培养液及1640培养液。用激光拉曼光谱仪进行检测。结果在585、633、656、674、707、772、776和798nm8个波长上胃癌患者和健康人均可以检测到重复性好的拉曼峰,前者峰值均高于后者,二者比较P<0.01,术后的拉曼峰明显低于术前,二者比较P值均<0.05,胃癌细胞体外培养液在上述8个波长处可观察到重复性好的拉曼峰。1640培养液在633、656和674nm3个波长处未检测到拉曼峰,胃癌细胞培养液的拉曼峰值与培养时间呈正相关。结论①胃癌患者血清拉曼光谱的峰值较健康人显著升高。②胃癌患者肿瘤切除手术后血清拉曼光谱的峰值较手术前明显下降。③胃癌细胞培养液拉曼峰值较1640培养液显著升高,随培养时间延长胃癌细胞培养液拉曼峰值逐渐升高。④激光拉曼光谱技术可检测出峰值的差异,可用于胃癌的筛查、预后监测及疗效判断的参考。[Objective] To study laser resonance Raman spectroscopy application in gastric cancer. [Methods] The specimens as follows were collected： the serum preoperation, pest-operation for three days of gastric cancer patients, nomal controls, the culture medium of SGC7901 gastric cancer cells cultured for 24, 48 and 72 hours and 1640 culture medium. [Results]At wavelength of 585 nm, 633 nm, 656 nm, 674 nm, 707 nm, 772 nm, 776 nm and 798 nm, the Raman peak of gastric cancer patients was higher than that of the normal （P 〈0.01）. Study of the Raman spectrum of gastric cancer patients preoperation and post-operation for three days shows that the Raman peak of pest-operation group was obviously lower than the Raman peak of pre-operation group. The numerical value of the P was 0.05 and the statistical difference is significant. From Raman spectroscopic study of the culture medium, the Raman peak repeatability at 585 nm, 633 nm, 656 rim, 674 nm, 707 nm, 772 nm, 776 nm and 798 nm could be found. But the Raman peaks at 633 nm, 656 nm and 674 nm can only be found in the culture medium of gastric cancer cells. The Raman peak was positively correlated with the culture time. [Conclusions] The Raman peak of gastric cancer patients was higher than that of the normal. The Raman peak of post-operation group was obviously lower than the Raman peak of pre-operation group.The technique Raman spectrum can reflect the differences of the spectrums. It is considered effctive for the screening of gastric cancer, the monitoring of prognosis and the judgment of therapeutic effect. The differences of the spectrums between the culture medium of gastric cancer cells and 1640 medium are significant. The Raman spectrum of culture medium of gastric cancer cells rises as time goes by.

关 键 词：胃癌 拉曼光谱 诊断 

分 类 号：R735.2[医药卫生—肿瘤] R730.49[医药卫生—临床医学]