HPLC测定蕨菜中的槲皮素和山萘酚  

Determination of quercetin and kaempferol in Pteridium aquilinumvar Latiusculum by HPLC

作　　者：李海丽[1] 

机构地区：[1]青海民族学院药学系,青海西宁810007

出　　处：《华西药学杂志》2009年第4期398-399,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用HPLC法测定蕨菜中的槲皮素和山萘酚。方法采用Agilent Zorbax SB-C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-0.04%磷酸（48：52）,流速1.0 m.lmin^-1,检测波长360 nm,柱温35℃。结果两种成分均达到基线分离,槲皮素和山萘酚的线性范围分别为0.029～0.200μg和0.0034～0.0119μg;r分别为0.9999和0.9998;平均回收率分别为102.7%（RSD=2.0%,n=6）和98.5%（RSD=1.7%,n=6）。结论所建方法快速、准确,可作为蕨菜药材质量控制的有效方法。OBJECTIVE To establish a quantitative method for the determination of quercetin and kaempferol in Pteridium aquilinumvarLatiusculum by HPLC.METHODS The samples were separated on the column of Agilent Zorbax SB-C18（250 mm×4.6 mm,5 μm） which was eluted with methanol and water（0.04 % phosphoric acid） in 48：52 with detected wavelength at 360 nm and flow rate at 1.0 ml·min^-1,column temperature at 35℃.RESULTS The results indicated that the two compounds were basically isolated.The linear ranges of quercetin and kaempferol were 0.029-0.20 μg（r=0.9999）,0.0034-0.0119 μg（r=0.9998） respectively.The average recoveries were 102.7%（RSD=2.0%,n=6）,98.5%（RSD=1.7%,n=6） respectively.CONCLUSION The method is rapid and precise.

关 键 词：高效液相色谱 槲皮素 山萘酚 蕨菜 

分 类 号：R917[医药卫生—药物分析学]