机械伤胁迫下刺梨果实的抗氧化反应  

Antioxidant Responses to Mechanical Wounding Stress of Rosa roxburghii Fruit

作　　者：黄伟[1] 安华明[1] 杨玉[1] 刘明[1] 

机构地区：[1]贵州大学喀斯特山地果树资源研究所,贵阳550025

出　　处：《西南大学学报（自然科学版）》2009年第8期109-113,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30660114);贵州省自然科学基金资助项目(20062038);贵州省优秀青年科技人才培养计划资助项目

摘　　要：以‘贵农五号’刺梨为材料,研究了刺梨果实对机械伤处理造成的氧化胁迫及其主要抗氧化系统的响应情况.结果表明,机械伤处理可诱导刺梨果实中O2.-和H2O2等活性氧的产生,促使膜脂过氧化水平加剧.在此过程中,抗氧化系统各组分的反应不尽相同:其中,抗坏血酸含量在处理2h内急剧降低,相应地其氧化态DHA的含量则持续增加;SOD活性在处理2h内急剧增强并一直保持较高水平,而抗氧化酶系统中的其它重要组分如APX、POD和CAT等却一直未能检测到活性.The oxidation stress in the fruit of thorn pear （Rosa roxburghii Tratt） caused by mechanical wounding and the responses of its main antioxidant systems were investigated with the variety ‘Guinong 5＇. The results showed that mechanical wounding stress induced the occurrence of the reactive oxygen species O2^＋- and H2O2 and enhanced membrane lipid peroxidation. In this process, the components of the antioxidant system responded differently： within 2 h of the mechanical wounding treatment, the content of ascorbic acid （AsA） decreased sharply and, accordingly, its oxidant state DHA content steadily increased; SOD activity increased rapidly and remained at a high level thereafter. But other important components of the antioxidant system such as APX, POD and CAT were not detected.

关 键 词：刺梨 机械伤胁迫 氧化胁迫 抗氧化反应 

分 类 号：Q949.751.8[生物学—植物学]