超声分子成像对小鼠肾脏急性微血管炎症的可视性评价  

Visible Evaluation of Mouse Acute Microvascular Inflammation in Kidney with Ultrasound Molecular Imaging

作　　者：陈少敏[1] 杨莉[3] 宾建平[2] 陈平[1] 吴爵非[2] 刘伊丽[2] 

机构地区：[1]汕头市中心医院中山大学附属汕头医院心内科,广东省汕头市515031 [2]南方医科大学南方医院心内科 [3]南方医科大学南方医院药学部

出　　处：《中国超声医学杂志》2009年第8期731-734,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家“863”计划项目（No.2006AA02Z478）

摘　　要：目的应用超声分子成像技术可视性评价小鼠肾脏急性微血管炎症。 方法缺血再灌注处理制备小鼠肾急性微血管炎症模型（IR组,6只）及肾假手术处理模型（SH组,6只）,所有实验小鼠均经静脉随机（间隔30min）弹丸注射携带抗小鼠P-选择素单抗靶向超声微泡（MBp）、同型对照抗体超声微泡（MBc）及普通脂质超声微泡（MB）,10min后行肾对比超声（CEU）检查,测量肾脏显影的声强度（VI）。并采用平行板流动腔技术在微血管生理血流条件下体外评价MBp的靶向黏附效能。 结果在缺血再灌注肾,MBp呈显著的超声显影,而MBc和MB呈轻度的超声显影;3种超声微泡在假手术肾均无明显的超声显影。MBp的VI值在缺血再灌注肾较假手术肾明显增大（P〈0.05）,同时较MBc和MB在缺血再灌注肾的VI值也都明显增大（P〈0.05）;而MBc及MB在缺血再灌注肾的VI值较各自假手术肾轻度增大（P〈0.05）。在微血管生理血流剪切应力环境下MBp可与小鼠P-选择素Fc段（PSFc）实现有效的靶向性特异结合。 结论超声分子成像技术可对肾脏急性微血管炎症进行有效的可视性评价,该技术将可用于可视性评价微血管炎症或相关的血管内皮反应。MBp在微血管生理血流条件下具有良好的靶向黏附效能。Objective To visibly evaluate mouse acute microvascular inflammation in kidney with ultrasound molecular imaging.Methods The model of renal ischemia-reperfusion（6 mice,group IR） and renal sham-operation（6 mice,group SH） in mice were established.（MBp）,（MBc） （MB）.ALL experimental mice accepted random venous injection（30 minutes interval） with anti-mouse P-selectin monoclone antibody（MBp）,ultrasound microbubbles with isotype control antibody of rat anti-mouse IgG MBc or and general lipid microbubbles MB,Renal video intensity（VI） was determined under renal contrast-enhanced ultrasound（CEU）10 minutes later.The targeting binding capability of MBp was assessed with parallel plate flow chamber under physiologic microvascular flow conditions in vitro.Results A significant enhancement of CEU imaging was observed in IR kidneys with MBp,while low-grade CEU imaging was observed in IR kidneys with MBc/MB.There was no markable CEU imaging in SH kidneys with MBp,MBc or MB.Compared to IR kidneys with MBc/MB and SH kidneys with MBp,significant increase in VI was observed in IR kidneys with MBp （P〈0.05）.There was respectively slightly increase of VI in IR kidneys with MBc/MB compared to SH kidneys with MBc/MB （P〈0.05）.At a shear stress of physiological microvascular blood flow,MBp can effectively bind with P-selectin.Conclusions Ultrasound molecular imaging can effectively and visibly evaluate acute microvascular inflammation in kidney and can be used to visibly evaluate microvascular inflammation and other endothelial responses.MBp has satisfactory targeting adhesion efficacy under physiological microvascular blood flow conditions.

关 键 词：靶向超声微泡 对比超声 分子成像 微血管炎症 P-选择素 

分 类 号：R730.4[医药卫生—肿瘤] R743.3[医药卫生—临床医学]