环氧化酶-2基因多态性与回族人群胃癌易感性的关联  

Correlation between the nucleotide polymorphisms of COX-2 and the susceptibility to gastric cancer in Hui ethnic group

作　　者：唐小凡[1] 李玉民[2] 李世雄[1] 李汛[1] 何文婷[1] 张丹[1] 张全保[1] 祝凯华[1] 

机构地区：[1]兰州大学第一医院普外二科,甘肃省兰州市730000 [2]兰州大学第二医院,甘肃省兰州市730000

出　　处：《世界华人消化杂志》2009年第17期1772-1776,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30870364~~

摘　　要：目的:探讨环氧化酶-2(COX-2)-765G>C的单核昔酸多态性与回族人群胃癌易感性的关系.方法:以PCR-限制性片段长度多态性方法对回族人群中胃癌组(n=100)、癌前病变组(n=102)和正常对照组(n=105)进行基因分型.使用ELISA法检测Hpylori感染.结果:COX-2-765GC+CC在胃癌组显著增高(P=0.021).交互作用分析显示:食腌菜的-765GC+CC携带者患胃癌的风险是不食腌菜的-765GG携带者的5.038倍(P=0.000);Hpylori感染阳性的-765GC+CC携带者患胃癌的风险是Hpylori感染阴性-765GG携带者的3.520倍(P=0.002).结论:COX-2-765G>C和中国甘肃地区回族人群胃癌易感性增高相关.-765GC+CC分别与食腌菜、Hpylori感染在胃癌的发病风险中存在着加乘交互效应.AIM： To explore the correlation between nucleotide polymorphisms in cyclooxygenase-2 （COX-2）-765G〉C and the susceptibility to gastric cancer in Hui ethnic group. METHODS： All investigated objects were divided into 3 groups： 100 gastric cancer patients, 102 precancerous lesion patients and 105 control subjects were enrolled. Polymerase chain reaction-restricted fragments length polymorphism （PCR-RFLP） method was used to detect genotypes of -765G〉C polymorphisms. ELASA method was used to identify IgGto H pylori. The results were analyzed using SPSS13.0 RESULTS： COX-2 -765GC ＋ CC genotypes were overrepresented in the gastric cancer group than in controls （P = 0.021）. Interaction analysis showed that -765 GC＋CC carriers who ate pickle vegetables a lot had a 5.038-fold increased risk for developing gastric cancer compared with -765GG carriers with not eating pickle vegetables （P = 0.000）; -765GC ＋ CC carriers with positive H pylori infection had a 3.520-fold increased risk for developing gastric cancer compared with -765GG carriers with negative H pylori infection （P = 0.002）. CONCLUSION： COX-2-765G〉C contributes to an increased susceptibility to gastric cancer in Hui ethnic group in Linxia City, Gansu Province. Multiplicative interactions exist between -765GC ＋ CC and eating pickle vegetables, -765GC ＋ CC and H pylori infection in the risk of developing gastric cancer.

关 键 词：胃癌 环氧化酶-2 基因多态性 

分 类 号：R735.2[医药卫生—肿瘤]