中国实验小型猪冠心病模型的心肌组织蛋白质组学研究  

Proteomics research on myocardial tissues of Chinese experimental mini-pig model with coronary heart disease

作　　者：苗兰[1] 刘建勋[1] 李欣志[1] 潘映红[2] 

机构地区：[1]中国中医科学院西苑医院,北京100091 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《中国药理学通报》2009年第7期880-883,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No90709001);国家"十一五"科技支撑计划资助项目(No2006BAI08B01-06)

摘　　要：目的研究中国小型猪冠心病模型心肌组织蛋白表达谱的改变情况。方法在建立中国小型猪冠心病模型基础上,应用双向电泳分离各组小型猪心肌组织总蛋白;通过图谱分析软件和基质辅助激光解析-飞行时间质谱技术分别进行差异比较和质谱鉴定及数据比对。结果小型猪冠心病模型组心肌组织与正常组相比共有14个差异蛋白点:13个上调点,1个特异表达点。这些差异点经胶内酶解、MAL-DI-TOF质谱分析及MASCOT搜索引擎检索,鉴定出HSPA8(Hsc70)、apolipoprotein A-IV(apoA-IV)、albumin及desmin4种蛋白。结论初步发现的差异蛋白可能与冠心病的发生、发展相关,但是还需要通过其它方法做进一步验证。Aim To explore the changes of protein expression pattern of myocardial tissues in mini-pig with coronary heart disease. Methods Based on the model of mini-pig with coronary heart disease, the total proteins of myocardial tissues from each group were separated by two dimensional gel electrophoresis （2-DE）. Protein exeprssion pattern in normal and model group was contrasted by hnage Master 2D Elite 6.01 analysis software. Then the different proteins were identified by MALDI-TOF-MS. Results Compared with normal group,14 proteins had been determined to change： 13 proteins were up-regulated and 1 proteins specifically expressed in mini-pig myocardial tissues of the dis- ease. With the search engine MASCOT, 4 proteins in- cluding HSPA8 （ Hsc70 ） protein, apolipoprotein A-IV （ apoA-IV ）, albumin and desmin were identified. Conclusions These four proteins may play an important role in the occurrence and development of coronary heart disease. The result will be further validated by other biological methods.

关 键 词：冠心病 心肌组织 蛋白质组学 中国实验小型猪 双向凝胶电泳 基质辅助激光解析-飞行时间质谱 

分 类 号：R-332[医药卫生] R322.11