Ghrelin对THP-1源性泡沫细胞ATP结合盒转运子A1和G1表达的调控作用  

Regulation of ghrelin on expression of ATP-binding cassette transporter A1/G1 in THP-1 derived foam cells

作　　者：万晶晶[1] 成蓓[1] 王彦富[1] 梅春丽[1] 刘玮[1] 柯丽[1] 何平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《中国病理生理杂志》2009年第8期1554-1558,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30471921)

摘　　要：目的:研究单核/巨噬细胞分化成为泡沫细胞过程中ghrelin对人ATP结合盒转运子A1和G1(ABCA1/ABCG1)表达的调控作用。方法:体外培养人源单核细胞系THP-1,由佛波酯(PMA)作用使其分化为巨噬细胞,后者可在氧化低密度脂蛋白(ox-LDL)存在条件下进一步转变为泡沫细胞。巨噬细胞加入不同浓度的Ghrelin预孵2h后再加入ox-LDL作用不同时间,油红O染色法观察细胞内脂滴含量,运用PT-PCR法检测ABCA1和ABCG1mRNA水平,Western blotting法检测ABCA1和ABCG1蛋白表达,采用酶法,通过荧光分光光度计检测细胞内外胆固醇含量并计算胆固醇流出率。结果:Ghrelin干预可明显减少细胞内脂滴的形成,显著增加胆固醇流出率,并能显著增加单核/巨噬细胞泡沫化过程中ABCA1和ABCG1mRNA水平和蛋白表达,且此作用呈浓度依赖性而不呈时间依赖性。结论:Ghrelin可能通过上调ABCA1和ABCG1转录翻译水平延缓动脉粥样硬化的发生。AIM ： To investigate the regulation of ghrelin on the expression of ATP - binding cassette transporter A1 and G1 （ABCA1/ABCG1）during the foam cell formation. METHODS： The human monocytie leukemia cell line （THP - 1 ） was chosen in our study. The differentiation of THP - 1 cells into macrophages was induced by using phorbol myristate acetate （PMA）. Macrophages were then incubated with oxidized LDL （ox -LDL）to generate foam cells. Ghrelin of different concentrations were treated at different time points during foam cell formation. The ABCA1/ABCG1 protein and mRNA levels were detected by Western blotting and RT - PCR. The effect of variance of cholesterol content was measured by zymochemistry via - fluorospectrophotometer. RESULTS ： Ghrelin reduced the content of lipid droplet in foam cells, and increased the efflux of intracellular cholesterol significantly. Ghrelin increased ABCA1 protein mass and mRNA level in dose - dependent manner. The changes of ABCG1 protein and mRNA level were the same as ABCA1. CONCLUSION： Ghrelin interfere atherosclerosis by up - regulating the expression of ABCA1 and ABCG1.

关 键 词：GHRELIN ATP结合盒转运子A1 ATP结合盒转运子G1 泡沫细胞 动脉硬化 

分 类 号：R363[医药卫生—病理学]