细胞因子在骨髓间充质干细胞修复小鼠急性肾衰竭中的作用  

Effects of cytokines on renovation of acute renal failure in mouse with bone marrow derived mesenchymal stem cell transplantation

作　　者：万建新[1] 郭琦[1] 王敬瀚[1] 潘阳彬[1] 崔炯[1] 傅槟槟[1] 许艳芳[1] 

机构地区：[1]福建医科大学附属第一医院肾内科,福建福州350005

出　　处：《中国病理生理杂志》2009年第8期1628-1634,共7页Chinese Journal of Pathophysiology

基　　金：福建省自然科学基金计划资助项目(No.C0710009)

摘　　要：目的:观察细胞因子在骨髓间充质干细胞(MSC)修复小鼠急性肾衰竭(ARF)中的作用。方法:用顺铂皮下注射建立ARF小鼠模型。小鼠随机分为正常对照组(control组)、急性肾衰竭组(ARF组)和骨髓间充质干细胞组(MSC组)。MSC组在建立ARF模型后24h经尾静脉输注小鼠MSC悬液。建模后第1、4、7、14、28d分批处死各组小鼠。测定血尿素氮(BUN)和血肌酐(Scr),观察肾组织病理变化,用RT-PCR和免疫组化方法检测肝细胞生长因子(HGF)、骨形态形成蛋白7(BMP-7)、肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)mRNA和蛋白的表达。结果:顺铂注射4d后,MSC组BUN、Scr值比ARF组明显降低(P<0.01),在第7和14d,仍可见MSC组的BUN、Scr水平低于ARF组(P<0.01,P<0.05)。MSC组小鼠肾小管损伤程度明显轻于ARF组。顺铂注射7d后,MSC组肾组织中BMP-7、HGF、IL-10mRNA和蛋白的表达均明显高于ARF组,而TNF-αmRNA和蛋白的表达则明显低于ARF组。结论:MSC可改善ARF小鼠肾功能,其机制可能与MSC促进肾脏局部生长因子分泌、减轻炎症反应有关。AIM ： To observe the effects of cytokines on renovation of acute renal failure （ARF） in mouse with bone marrow derived mesenchymal stem cell （MSC） transplantation. METHODS： ARF animal model was induced in mouse by subcutaneous injection of cisplatin. Mice were randomly assigned into 3 groups： normal control group, ARF group and MSC group. After 24 h cisplatin injection, animals were injected intravenously with MSC in MSC group. Animals were sacrificed at 1 d, 4 d, 7 d, 14 d and 28 d after cisplatin injection. The blood urea nitrogen （BUN） and serum ereatinine （Scr） were measured. The renal morphologic changes were scored with Paller＇s criterion on hematoxylin and eosin （HE） stained seetions. The mRNA and protein expressions of HGF, BMP-7, TNF -α and IL- 10 were detected by RT - PCR and immunohistochemistry method. RESULTS ： After 4 d of eisplatin injection, the BUN and Ser values in MSC group were significantly lower than those in ARF group （ P 〈 0. 01 ）. After 7 d and 14 d, the values of BUN and Set in MSC group were still lower than those in ARF group （ P 〈 0. 01, P 〈 0. 05 ）. The renal morphologie scores of MSC group were also lower than those of ARF group. After 7 d, the expressions of HGF, BMP -7 and IL - 10 were higher in MSC group than those in ARF group, the expression of TNF -α in MSC group was lower than that in ARF group. CONCLUSION： MSC promotes the recovery of acute renal failure induced by cisplatin. The mechanism may partly depend on paraerine of growth factor and amelioration of inflammatory.

关 键 词：肾功能衰竭 急性 骨髓间充质干细胞 细胞因子类 

分 类 号：R363[医药卫生—病理学]