Cloning and Bioinformatics Analysis of Lfcin Gene of Datong Yak  

“大通”牦牛Lfcin基因克隆及生物信息学分析(英文)

作　　者：裴杰[1] 阎萍[1] 姬国红[2] 冯瑞林[1] 梁春年[1] 郭宪[1] 曾玉峰[1] 包鹏甲[1] 褚敏[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [2]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《Agricultural Science & Technology》2009年第3期50-54,共5页农业科学与技术（英文版）

基　　金：Supported by Project of Basic Research Fund for National Non-profit Institute of China (BRF070105)~~

摘　　要：[Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. [Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, dairy cow, human and mouse were used for sequence alignment and phylogenesis analysis. [Result] The second exon of lactoferrin(LF) from Datong yak, which is 778 bp in length, was obtained, within which the coding region of Lfcin gene is 75 bp (25 amino acid residues); sequence analysis showed that there is discrepancy of eleven bases between Datong yak and dairy cow; Lfcin proteins from various species shared high homeology, of which that from Datong yak and dairy cow were completely identical; phylogenesis analysis showed that cladogram based on Lfcin was consistent with species evolutionary law. [Conclusion] This study laid a foundation for the prokaryotic or eukaryotic expression of Lfcin gene and further understanding the activity of Lfcin protein.[Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. [Method] Using PCR technology, the lactoferricin（Lfcin）-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, dairy cow, human and mouse were used for sequence alignment and phylogenesis analysis. [Result] The second exon of lactoferrin（LF） from Datong yak, which is 778 bp in length, was obtained, within which the coding region of Lfcin gene is 75 bp （25 amino acid residues）; sequence analysis showed that there is discrepancy of eleven bases between Datong yak and dairy cow; Lfcin proteins from various species shared high homeology, of which that from Datong yak and dairy cow were completely identical; phylogenesis analysis showed that cladogram based on Lfcin was consistent with species evolutionary law. [Conclusion] This study laid a foundation for the prokaryotic or eukaryotic expression of Lfcin gene and further understanding the activity of Lfcin protein.

关 键 词：YAK Lfcin gene CLONE BIOINFORMATICS 

分 类 号：S823[农业科学—畜牧学]