生物膜铜绿假单胞菌AmpC基因诱导表达的研究  

The induced expression of AmpC gene in pseudomonas aeruginosa bioflims

作　　者：赵京明[1] 成炜[1] 蒋捍东[1] 

机构地区：[1]青岛大学医学院附属医院呼吸科,266003

出　　处：《中国医药》2009年第9期641-643,共3页China Medicine

基　　金：山东省医药卫生科技发展计划项目（2005HZ020）

摘　　要：目的研究铜绿假单胞菌的产酶基因AmpC在浮游和生物膜状态下的表达差异。方法改良的平板法建立铜绿假单胞菌生物膜模型，抗生素诱导浮游菌和生物膜菌AmpC基因表达，实时荧光定量聚合酶链反应测定PA01的AmpC基因表达水平。结果抗生素诱导前，PAO1浮游菌和生物膜菌的AmpC基因表达均较低，诱导后AmpC基因表达均明显上调。运用产最大AmpC酶活性浓度的抗生素诱导后，亚胺培南和头孢他啶诱导的生物膜菌的AmpC基因表达量高于其诱导的浮游菌。在浮游和生物膜状态下，亚胺培南诱导PAO1的AmpC基因表达量均高于头孢他啶。结论生物膜PA较浮游茵更易被诱导产生AmpC酶，亚胺培南的诱导能力高于头孢他啶。Objective To study the expression of AmpC gene in Pseudomonas aeruginosa induced by antibiotics at plank tonic and biofilms phases. Methods An in vitro model of PAOI biofilms was established with modified flat-board method. Being induced by antibiotics at plank tonic and biofilms phases, the expression of AmpC gene in PAOI was quantified by real-time quantitative PCR. Results Without the effect of antibiotics,the expression of AmpC gene in PAO1 was low at both plank tonic and biofilms phases. Being induced by imipenem and ceftazidime that can induce the maximal AmpC β-lactamase activities, the expression of AmpC gene in biofilms PAO1 was higher than that in plank tonic PAOI. During both plank tonic and biofdms phases, the expression of AmpC gene in PAO1 induced by imipenem was higher than that induced by ceftazidime. Conclusion Induced by antibiotics,biofilms PAO1 showes stronger ability for the expression of AmpC gene than plank tonic PAOI. The expression of AmpC gene induced by imipenem is higher than that induced by ceftazidime.

关 键 词：铜绿假单胞菌 生物膜 AMPC 基因 实时荧光定量聚合酶链反应 

分 类 号：R378.991[医药卫生—病原生物学]