游离脂肪酸激活HaCaT细胞表面TLR对NF-ΚB信号转导通路的影响  

The infact of NF-ΚB signal transduction by active Toll-like receptors on the surface of HaCaT cells

作　　者：韩勇彬[1] 胡大海[2] 高宗科[1] 黄松涛[1] 周骥[1] 樊萍[1] 李艳秋[1] 

机构地区：[1]中国人民解放军第537医院,陕西宝鸡721006 [2]第四军医大学西京医院全军烧伤中心,陕西西安710032

出　　处：《中国美容医学》2009年第8期1128-1130,共3页Chinese Journal of Aesthetic Medicine

摘　　要：目的:观察长链游离脂肪酸对体外培养的角质形成细胞的作用,探讨其对TLR及NF-ΚB信号转导通路的影响。方法:①体外培养Hacat细胞,实验分为N、PA、SA、LPS四组,N组为正常对照组,软脂酸(Palmitic Acid,PA)750μmol/L、硬脂酸(Stearic Acid,SA)750μmol/L、脂多糖(lipopolysaccharide,LPS)500 ng/L和Hacat细胞共培养72h组分别为N组、PA组、SA组、LPS组;②LPS、SA与角质形成细胞共培养72h检测LPS、SA对Hacat细胞NF-κB、p-IκBα、IκBα表达的影响。通过免疫印迹法(Weston blot)检测SA、LPS对Hacat细胞TLR2、TLR4、NF-ΚB、p-IκBα、IκBα表达的影响;Real Ti me PCR方法检测对Hacat细胞内NF-κB、TNF-αmRNA表达的影响。结果:SA、LPS激活细胞表面TLR后可以明显促进细胞表面TLR2、TLR4表达及细胞内NF-κB、TNF-α等促炎症因子的表达。PA促进Hacat细胞内NF-ΚB、p-IκBα、IκBα表达无明显影响(P>0.05)结论:SA可促进角质形成细胞分泌NF-κB、TNF-α等因子表达促进炎症反应;其机制可能为:SA可能通过TLR及NF-κB信号传导通路促进细胞释放NF-κB、TNF-α等促炎因子。Objective To investigate the mechanism of free fatty acids （FFA） induced Toll-like receptors activation on the Hacat cell surface to promote Tissue-Repair. Methods Free fatty acids （FFA） and Hacat cell were cultured and divided into N,PA,SA and LPS groups. Group N as control, Palmitic Acid （PA） 750μmol/L, Stearic Acid （SA） 750μmol/L and lipopolysaccharide （LPS）500ng/L were cultured with Hacat cells 72h which were divided into N,PA,SA and LPS groups. NF-κB,p- IκBα, IκBα, TLR2 and TLR4 were detected by Western blot. NF-κB and TNF-α were detected by real-time PCR. Results stearic acid （SA） and LPS can active Toll-like receptors on the surface of Hacat cells to promote the expression of NF-κB and TNF-α. There wasn＇t significant difference in the insulin level of NF-κB and TNF-α between control and Palmitic Acid （PA） groups. Conclusion Stearic acid （SA） may active Toll-like receptors on the surface of Hacat cells to promote NF-κB signal transduction.

关 键 词：游离脂肪酸 HACAT细胞 TOLL-LIKE receptor NF-κB炎症反应 

分 类 号：Q813.1[生物学—生物工程]