玻璃体切割术后晶状体酶活性的变化  

Changes of enzyme activities in lens after vitrectomy

作　　者：刘晓萃[1] 王萍[1] 严宏[1] 

机构地区：[1]中国人民解放军第四军医大学唐都医院眼科,西安710038

出　　处：《眼科研究》2009年第8期645-648,共4页Chinese Ophthalmic Research

基　　金：国家自然科学基金资助(30872837)

摘　　要：目的观察玻璃体切割术后晶状体酶活性的变化,探讨玻璃体切割术后白内障的发病机制。方法新西兰白兔8只(16只眼)分为2组,左眼为玻璃体切割组,行经睫状体扁平部次全玻璃体切割术,右眼为正常对照组。定期观察晶状体混浊情况,5个月后取出晶状体行Na+-K+-ATP酶、过氧化氢酶和谷胱甘肽还原酶活性检测。结果5个月后,玻璃体切割组晶状体在透明度和组织形态学上与正常对照组差异无统计学意义,玻璃体切割组与正常对照组相比,Na+-K+-ATP酶、过氧化氢酶和谷胱甘肽还原酶活性分别下降了56%、17%和5%,差异均有统计学意义(P<0.05)。结论玻璃体切割术可使成年兔晶状体发生生物化学改变,对研究玻璃体切割术后白内障的发生发展机制有重要的指导意义。Objective Vitrectomy is a commen treating approach to multiple kinds of vitreoretinopathy and ocular trauma. However,metabolism of lens protein often alter following vitrectomy. Reseach showed the incidence of lens opacity was 68% - 98% after vitrectomy. Present study was to investigate the enzyme activity in lens after vitrectomy and study the mechanism of post-vitreetomy cataract. Methods Sixteen eyes of eight New-Zealand rabbits （2.3 - 2.4 kg） were divided into two groups. The left eyes of rabbits underwent standard three port pars plana vitrectomy to remove all visible vitreous gel,and the normal right eyes served as controls. Transparency of lens was monitored by a slit lamp biomieroscopy before and after vitrectomy. The morphology of lens cells was observed under the light microscope. The activities of Na^＋ -K^＋ -ATPase, glutathione reductase （GR） , catalase in lenses were detected five months after vitreetomy. The experimental procedure of animals followed the Standard of Association for Reaseareh in Vision and Ophthalmology. Results Lenses were clear in both operation group and normal control group from one month through five months after operation. The morphology and structure of lens cells were normal under the light microscope in both operation group and normal group at the fifth month after operation. The activity of Na^＋ -K^＋-ATPase was significantly decreased in operation group compared with control group （ 0. 024 9 ± 0. 004 7 versus 0. 057 2 ± 0. 008 9, t = 7. 872,P=0.004）,and so was the catalase （2.0395 ± 0. 156 versus 2. 4744±0. 100 2,t = 5. 745,P = 0.000） and GR （2. 650 8±0. 089 8 versus 2. 789 1±0. 081 7,t =2. 791 ,P =0. 019） between operation group and control group. The activities of Na^＋-K^＋ -ATPase,catalase and GR declined by 56% ,17% and 5% respectively in comparison with control group. Conclusion Vitrectomy can induce the change of Na^＋ -K^＋ -ATPase,catalase and GR in lens in rabbit.

关 键 词：玻璃体切割术 晶状体 NA+-K+-ATP酶 过氧化氢酶 谷胱甘肽还原酶 

分 类 号：R779.6[医药卫生—眼科]