薏苡仁诱导急性T淋巴细胞白血病Jurkat细胞凋亡及其机制  

Coix Lachrymajobi L Varma-Yuan Induces Apoptosis of Jurkat Cell Line in Acute T Lymphoblast Leukemia and Its Mechanism

作　　者：姚根宏[1] 张国栋[2] 栾建凤[1] 叶东[1] 严京梅 朱培元[1] 雷千红[1] 

机构地区：[1]南京军区南京总医院南京大学医学院临床学院输血科,江苏南京210002 [2]南京医科大学临床学院,江苏南京210029

出　　处：《中国实验血液学杂志》2009年第4期879-882,共4页Journal of Experimental Hematology

基　　金：南京军区南京总医院科研基金(编号M2008067);中国博士后基金(编号20060390940)资助

摘　　要：本研究探讨薏苡仁油对急性T淋巴细胞白血病Jurkat细胞的增殖和凋亡效应及其作用机制。分别用0.4,0.8,1.6mg/ml的薏苡仁油作用Jurkat细胞24小时,用CCK法检测细胞增殖,应用Hoechst33258染色、PI及PI/Annexin V染色后流式细胞仪来检测细胞凋亡,然后采用JC-1染色分析线粒体膜电位。结果表明:0.8和1.6mg/ml的薏苡仁油能显著抑制Jurkat细胞增殖;随着薏苡仁油浓度的增加,凋亡细胞数目增多,线粒体膜电位降低。结论:薏苡仁油对急性T淋巴细胞白血病Jurkat细胞有明显的抗增殖和促凋亡作用,而且此效应与线粒体膜电位降低有关,本研究结果为临床应用薏苡仁油治疗白血病提供了实验依据。The aim of the present study was to investigate the anti-proliferation and pro-apoptosis effect of Coix lachrymajobi L varma-yuan on acute T lymphoblast leukemia cell line Jurkat cells and its mechanism. Jurkat cells were treated with Coix lachrymajobi L varma-yuan of various concentrations （0, 0.4, 0.8, 1.6 mg/ml） for 24h. The inhibitory ratio was measured by Cell Counting Kit-8. The effects of Coix lachrymajobi L varma-yuan on apoptosis of Jurkat cells were determined by Hoechst 33258,PI and Annexin V-FITC/PI double staining. The mitochondrial membrane potential was analyzed by JC-1 staining. The results demonstrated that Coix lachrymajobi L varma-yuan inhibited the proli-feration of Jurkat ceils, and induced chromatin condensation and fragmentation （ characteristic of apoptosis） and loss of mitochondrial membrane potential. In conclusion, Coix lachrymajobi L varma-yuan can inhibit the cell proliferation and induce the apoptosis of Jurkat cells. These effects relate to loss of mitochondrial membrane potential. These results suggest that Coix lachrymajobi L varma-yuan may be of value in treating lymphoma.

关 键 词：薏苡仁油 急性T淋巴细胞白血病 JURKAT细胞 细胞凋亡 线粒体膜电位 

分 类 号：R733.71[医药卫生—肿瘤] R979.1[医药卫生—临床医学]