碳纤维超微盘电极的制备及对单囊泡胞吐动力学的高分辨研究  

Fabrication of Ultra-micro Disk Carbon Fiber Electrode that Enables Monitoring Single Vesicle Release Kinetics with High Sensitivity

作　　者：李雷[1] 李照玉[1] 万敏[1] 张蓉颖[1] 

机构地区：[1]分子生物物理教育部重点实验室,华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《分析科学学报》2009年第4期378-382,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.30500117);科技部国家重大科学研究计划蛋白质专项(No.2007CB914200);湖北省自然科学基金(No.2005ABA045)

摘　　要：利用改良的电化学腐蚀方法制备了碳纤维超微盘状电极,具有方法简便,电极尺寸可控、可多次使用等优点。扫描电镜和伏安表征实验表明电极密封效果良好、电化学性能优良。在鼠嗜铬神经瘤PC12细胞上,用超微盘电极实现了高时空分辨、高保真度的单囊泡胞吐测定。与常规微电极相比,能更准确地反应囊泡融合这一超快过程(毫秒级)的动力学过程。Carbon ultramicroelectrode has attractive potential application in probing neurotransmitter release at synapse terminals and fusion pore dynamics. In this study, we reported a facile method of fabricating ultra-micro disk carbon fiber electrodes （CFEs） using a refined electrochemical etching and plastic insulation techniques. The fabricated disk electrodes show ideal steady-state voltammetric behaviors, and SEM micrographs show the tips are fine ultra-micro disk, while the geometry of electrodes is fairly uniform cylinder, which enables repetitious usage of the electrode upon recutting of the tip 2-3 times. The ultra-micro disk CFEs are also shown to be ideal for probing the vesicle fusion dynamics due to its high spatial resolution, which enables only the secretion events occurred subjacent to the electrode to be detected.

关 键 词：超微盘电极 电化学检测 囊泡 胞吐 融合动力学 

分 类 号：O657.1[理学—分析化学]