聚对氨基苯磺酸修饰玻碳电极不可逆双安培法测定酪氨酸  

Determination of Tyrosine Based on the Poly(p-aminobenzene sulfonic acid) Modified Glassy Carbon Electrode by Irreversible Biamperometry

作　　者：李利军[1] 钟亮[2] 蔡卓[2] 程昊[1] 喻来波[2] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]广西大学化学与化学工程学院,广西南宁530004

出　　处：《分析科学学报》2009年第4期435-438,共4页Journal of Analytical Science

基　　金：桂科自(No.0640029);桂科自(No.0832062)

摘　　要：在玻碳电极上成功地制备了聚对氨基苯磺酸修饰电极（P-p-ABSA／GCE），研究了酪氨酸在该修饰电极上的电化学行为。将此修饰电极用于流动注射不可逆双安培体系的构建，建立了流动注射双安培法直接测定酪氨酸的方法。在0V外加电压下，在0．005mol／L硫酸载液中，酪氨酸的氧化峰峰电流与其浓度在2．0×10^-6 ～2．0×10^-4 mol／L范围内呈良好的线性关系，方法的检出限（S／N-3）为1．0×10^-7 mol／L。连续测定1．00×10^-5 mol／L的酪氨酸标准溶液，电流值的相对标准偏差（RSD）为1．48％（n=20）。该方法具有较高的选择性和灵敏度，应用于测定复方氨基酸注射液中酪氨酸的含量的测定，结果比较满意。A poly（p- aminobenzene sulfonic acid） modified glassy carbon electrode（GCE） was fabricated and the electrochemical behavior of tyrosine （Tyr）on this modified electrode was studied. The hybrid thin film modified electrode was applied to the flow-injection irreversible biamperometric analysis. The analysis system was composed of the oxidation of Tyr at a P-p-ABSA/GCE and the reduction of permanganate at an another bare gold electrode. The flow injection irreversible biamperometry analysis method was studied under the applied potential difference of 0 V. In the 0.005 mol/L sulfuric acid, the oxidative peak current increased linearly with the concentration of Tyr in the range of 2.0× 10^-6 -2.0× 10^-4 mol/L. The detection limit for Tyr was 1.0 × 10^-7 mol/L（S/N=3） and the RSD for 20 replicate determination of 1.00 N 10^-5 mol/L Tyr was 1.48%. The method was applied for the analysis of Tyr in injection with satisfactory results.

关 键 词：玻碳电极 不可逆双安培法 修饰电极 酪氨酸 

分 类 号：O657.1[理学—分析化学]