高效液相色谱-荧光检测大豆异黄酮中的苯并[a]芘  

Study on HPLC-FLD Analysis of Benzo[a]pyrene in Soybean Isoflavone

作　　者：周牡丹[1] 程冬萍[1] 何国勇[1] 陈鸳谊[1] 丁鼎[1] 颜继忠[1] 

机构地区：[1]浙江工业大学药学院,杭州310014

出　　处：《食品工业》2009年第4期71-73,共3页The Food Industry

摘　　要：建立大豆异黄酮中苯并[a]芘残留的高效液相色谱测定法。采用hypersil ODSC18分析柱(25μm,250mm×4.6mmi.d);流动相:甲醇∶水(90∶10);流速1.0mL/min;激发波长297nm、发射波长410nm;样品进样量20μL;柱温35℃;保留时间的相对标准偏差0.816%;方法的检出限0.1μg/kg;在三个添加浓度水平上的平均回收率分别为98.16%、87.76%、78.86%,三次测定的相对标准偏差0.45%、1.63%、0.57%。该法样品预处理简单快速,无需将样品过柱净化,分析成本大大降低,具有毒性小,灵敏度高,分析时间短等优点。A method for determination of benzo[a]pyrene in soybean isoflavone by HPLC-FLD was developed for the first time. In the method, the analysis column is revered-phase column of C188 （25 μm, 250 mm×4.6 mm i.d）, the mobile phase is methanol/water （90：10）, the flow is 1.0 mL/min, excitation wavelength is 297nm, emission wavelength is 410 nm, the column temperature is 35℃, and the injection volume is 20 μL. The RSD of retention time was 0.816%. The limit of detection was 0.1 μg/kg. The average recoveries of three different addition levels were 98.16%, 87.76% and 78.86%, precision RSD of the three detections were 0.45%, 1.63%, 0.57%. The method is rapid, cheap, safe and sensitive for directly determining benzo [a] pyrene in soybean isoflavone.

关 键 词：苯并[A]芘 高效液相色谱仪 大豆异黄酮 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]