肉桂酸甲酯抑制酪氨酸酶催化反应的动力学研究  

Kinetics of the inhibition of methyl cinnamate on tyrosinase catalyzed reaction

作　　者：龚盛昭[1] 王晓立[1] 林取妹[1] 高晓慧[1] 

机构地区：[1]广东轻工职业技术学院轻化工程系,广东广州510300

出　　处：《化学研究与应用》2009年第8期1168-1172,共5页Chemical Research and Application

基　　金：广东省科技计划项目(2007B080701004)

摘　　要：The inhibitory effects of methyl cinnamate on the monophenolase activity and diphenolase activity of tyrosinase were studied by enzymological kinetic method in Na2HPO4-NaH2PO4 buffer solution(pH=6.8) at 30 ℃.Methyl cinnamate was found to inhibit the monophenolase activity and diphenolase activity of tyrosinase.The methyl cinnamate concentration leading to 50 % inhibitory rate(IC50) were 0.61 mmol/L for monophenolase activity and 1.49 mmol/L for diphenolase activity,respectively.Methyl cinnamate coued extend the lag time of monophenolase.0.8 mmol/L of methyl cinnamate resulted in the lag time extension from 1.1 min to 3.2 min.The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that methyl cinnamate was a noncompetitive inhibitor to diphenolase,and the inhibition constant KI for inhibitor binding with enzyme(E)was 0.66 mmol/L.The inhibitory effects of methyl cinnamate on the monophenolase activity and diphenolase activity of tyrosinase were studied by enzymological kinetic method in Na2HPO：NaH2PO4 buffer solution （pH = 6. 8） at 30 ℃. Methyl cinnamate was found to inhibit the monophenolase activity and diphenolase activity of tyrosinase. The methyl cinnamate concentration leading to 50 % inhibitory rate （ 1C50 ） were 0.61 mmol/L for monophenolase activity and 1.49 mmol/L for diphenolase activity, respectively. Methyl cinnamate coued extend the lag time of monophenolase. 0.8 mmol/L of methyl cinnamate resulted in the lag time extension from 1.1 min to 3.2 min. The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that methyl cinnamate was a noncompetitive inhibitor to diphenolase, and the inhibition constant K1 for inhibitor binding with enzyme （E）was 0.66 mmol/L.

关 键 词：酪氨酸酶 肉桂酸甲酯 抑制作用 动力学 单酚酶 二酚酶 

分 类 号：Q356.1[生物学—遗传学] O643.1[理学—物理化学]