产1,3-丙二醇重组大肠杆菌JM109(pEtac-dha B-tac-yqh D)的构建  

Construction of recombinant Escherichia coli for producing 1, 3-propanediol

作　　者：张成[1] 诸葛斌[1] 方慧英[1] 诸葛健[1] 

机构地区：[1]江南大学工业微生物研究中心江南大学工业生物技术教育部重点实验室,无锡214122

出　　处：《工业微生物》2009年第4期7-11,共5页Industrial Microbiology

基　　金：国家"863"高新项目资助(NO.2006AA020103)

摘　　要：在生物柴油的生产过程中,最高可得到约10%的副产物甘油,副产物甘油的去向将成为生物柴油大规模产业化发展所面临的严峻问题。以生物柴油副产物甘油为原料耦合生产1,3-丙二醇,不仅解决了生物柴油副产物甘油的出路问题,同时降低了1,3-丙二醇的生产成本。本研究在前期工作的基础上,分别获得了来源于肺炎克雷伯氏菌的甘油脱水酶编码基因dhaB和来源于大肠杆菌的1,3-PD氧化还原酶同工酶编码基因 yqhD,利用表达载体pEtac串联构建了重组质粒pEtac-dhaB-tac-yqhD,将其转化大肠杆菌得到产1,3-丙二醇重组大肠杆菌JM109(pEtac-dhaB-tac-yqhD),降低了代谢中间产物3-羟基丙醛的积累,提高了1,3-丙二醇的产量。Glycerol as a by-product is as high as about 10% of biodiesel during the process of biodiesel production. How to convert glycerol becomes a serious problem to be resolved if considering large amount of biodiesel production. The integrated production of biodiesel and 1, 3-propanediol can solve the utilization of by-product glycerol of biodiesel production and reduce the cost of 1, 3-propanediol production. In the previous study, gene dhaB encoding for glycerol dehydratase from Klebsiella pneumoniae and gene yqhD encoding for 1, 3-propanediol oxidoreductase isoenzyme from Escherichia coli were cloned, respectively. In this study, gene dhaB and gene yqhD were transferred into expression vector pEt ac to construct recombinant E. coli JM109 （pEtac-dha B-tac-yqhD）. It could decreased the accumulation of 3- hydroxypropionaldehyde and increased the production of 1, 3-PD.

关 键 词：生物柴油 甘油 重组大肠杆菌 1 3-丙二醇 

分 类 号：TQ223.162[化学工程—有机化工] TQ920.1[轻工技术与工程—发酵工程]