高效液相色谱法测定麦芽糖含量  

Determination of Maltose Contents by HPLC

作　　者：张雪荣[1] 池利民[1] 李素霞[1] 

机构地区：[1]河北化工医药职业技术学院,河北石家庄050026

出　　处：《安徽农业科学》2009年第24期11343-11344,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立麦芽糖的含量测定方法。[方法]采用高效液相色谱法(HPLC),色谱柱为Alltima氨基柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(62∶38),流速为1.3 ml/min。[结果]在选定的试验条件下,Alltima氨基柱的理论塔板数为2 855,麦芽糖峰与杂质峰的分离度为6.09,精密度试验的RSD为0.90%(n=6)。[结论]该法测定麦芽糖含量具有简捷、实用、准确的特点。[ Objective ] The determination method of Maltose contents were established. [ Method ] HPLC method was used, chromatographic column was the Alltima amido-column （250 mm × 4.6mm,5μm） ,mobile phase was aeetonitrile-water （62：38）, flowing rate of 1.3 mL/min. [ Result ] Under the selected tested conditions,theoretical plate number of the Alhima amido-column was 2 855 ,the separation degree of Maltose and impurity peak were 6.09,RSD was 0.90% （n =6）. [ Conclusion] The method was proved to be simple,practical and accurate .

关 键 词：HPLC 麦芽糖 含量测定 

分 类 号：O657.7[理学—分析化学]