基于三维荧光光谱法的水样中苯酚和对苯二酚含量测定  

Direct Determination of Phenol and Hydroquinone Contents in Environmental Water Based on Three-dimensional Fluorescence Spectroscopy

作　　者：崔香[1] 郭幼敬[2] 常敏 

机构地区：[1]青海师范大学化学系,青海西宁810008 [2]青海师范大学实验管理中心,青海西宁810008 [3]陕西省米脂县杨家沟中学,陕西米脂718109

出　　处：《安徽农业科学》2009年第24期11351-11352,11355,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究用荧光分光光度法测定水中苯酚和对苯二酚的含量,为环境样品中痕量物质的分析提供测定方法。[方法]取水样,加入β-环糊精溶液、EDTA溶液及缓冲溶液后测定。[结果]在波长对为λEx/λEm=240 nm/309 nm时测定苯酚含量,线性范围为0～1×10-4mol/L,检出限为6.6×10-8mol/L;在波长对为λEx/λEm=310 nm/340 nm时测定对苯二酚含量,线性范围为0～1.5×10-5mol/L,检出限为5.2×10-9mol/L。[结论]通过三维荧光扫描来选择干扰少而测定灵敏度较高的检测波长,实现了荧光分光光度法直接测定水样中的苯酚和对苯二酚含量。[ Objective ] The research aimed to measure phenol and hydroquinone contents in water by fluorescence speetrophotometry, provide a new method to analyze trace substances in environmental samples. [ Method ] The method firstly sampled water and added β-eyelodextrin and EDTA buffer solution in it, then the measurement was carried out. [ Result] Phenol were tested when wave length was at λEx/λEm=240nm/309 nm. The results showed that the linear range was 0 - 1 × 10^-4 mol/L and the detection limit was 6.6× 10^-5 mol/L. Hydroquinone was tested when wave length was λEx/λEm= 310 nm/340 nm. The results showed that the linear range was 0 - 1.5 × 10^-5 mol/L and the detection limit was 5.2 × 10^-9 mol/L. [ Conclusion] The method of choose detection wave length with higher sensitivity and less interference by three-dimensional fluorescence scanning allows to determine directly the phenol and hydroquinone content by fluorescence speetrophotometry in water samples.

关 键 词：荧光分光光度法 苯酚 对苯二酚 Β-环糊精 三维荧光 

分 类 号：O652[理学—分析化学]