细胞因子IL-2和IL-6对胰腺癌细胞表达VEGF-D的调节  

Cytokines IL-2 and IL-6 Regulate Expression of VEGF-D in Pancreatic Cancer Cells

作　　者：唐瑞峰[1] 齐帅[1] 苑建磊[1] 孙超[1] 田贵金[1] 李智峰[1] 张志明[1] 

机构地区：[1]河北医科大学第四医院肝胆外科,石家庄050011

出　　处：《肿瘤防治研究》2009年第8期651-654,共4页Cancer Research on Prevention and Treatment

基　　金：河北省研究与发展科技攻关计划项目(05276101D-71)

摘　　要：目的探讨细胞因子白细胞介素2(IL-2)和白细胞介素6(IL-6)对胰腺癌细胞表达VEGF-D的调节。方法以细胞因子IL-2或IL-6分别刺激胰腺癌细胞株SW1990和BXPC-3后用逆转录-聚合酶链式反应技术(RT-PCR)分析其VEGF-D基因的表达。结果IL-2使细胞株SW1990和BXPC-3产生VEGF-D mRNA减少;IL-6使细胞株SW1990产生VEGF-D mRNA增加,但对细胞株BXPC-3产生VEGF-D mRNA无明显影响。结论IL-2抑制胰腺癌细胞VEGF-D mRNA的表达,从而抑制胰腺癌淋巴结转移;IL-6促进某些胰腺癌细胞VEGF-D mRNA的表达,但对胰腺癌细胞生物学特性的影响有待于进一步深入研究。Objective To explore the function of cytokines interleukin-2 （IL-2） and interleukin-6 （IL-6） on regulating the expression of vascular endothelial growth factor D（VEGF D） in cultured human pancreatic cancer cell lines. Methods We used reverse transcription polymerase chain reaction（RT-PCR） methods to anaylyze VEGF-D mRNA level in two cultured human pancreatic cancer cell iines（SW1990 and BXPC- 3） under the stimulation with IL-2 or IL-6. Results RT-PCR analysis revealed the expression of VEGF- D gene was decreased in SW1990 cell or BXPC-3 cell stimulated by IL-2. IL-6 up-regulated VEGFD mR- NA of SW1990 cell, the in consistency-dependent, but there was no effect on VEGF-D mRNA expression of BXPC-3 cell line. Conclusion IL-2 inhibits lymph node metastasis of pancreatic cancer by inhibiting expression of VEGF-D; IL-6 increases expression of VEGF-D mRNA in several pancreatic cancer cell, but the effects on biological characteristic of pancreatic cancer cell still need to be continuing studied.

关 键 词：胰腺癌 白细胞介素2 白细胞介素6 血管内皮生长因子D 逆转录-聚合酶链式反应 

分 类 号：R735.9[医药卫生—肿瘤]