羊布鲁菌双抗体夹心ELISA检测方法的建立及验证  

Development and Verification of Double Antibody Sandwich ELISA for B.melitensis

作　　者：梅建军[1] 王兴龙[2] 李晓燕[2] 刘锴[2] 任林柱[2] 贾剑锋 张琴 

机构地区：[1]内蒙古包头医学院基础医学部,内蒙古包头014010 [2]军事医学科学院军事兽医研究所,长春130062 [3]内蒙古乌拉特中旗人民医院,内蒙古乌拉特中旗015300

出　　处：《中国生物制品学杂志》2009年第8期823-825,共3页Chinese Journal of Biologicals

摘　　要：目的建立羊布鲁菌双抗体夹心ELISA检测方法,并进行验证。方法应用针对羊布鲁菌O链M抗原的种特异性单克隆抗体,建立检测羊布鲁菌抗原的双抗体夹心ELISA方法,并进行重复性、特异性、敏感性验证及初步应用。结果经验证,该方法重复性好,特异性强,最低检出限为3.0×103CFU/ml,与平板凝集试验的符合率为100%。结论已建立了特异、敏感的检测羊布鲁菌的双抗体夹心ELISA方法。Objective To develop an ELISA method for detection of B. melitensis. Methods A double antibody sandwich ELISA method was developed with specific antibody against M antigen of B. melitensis O chain, then verified for reproducibility, specificity and sensitivity, and applied primarily. Results The developed ELISA method showed good reproducibility and high specificity, and its minimum detection limit was 3. 0 ×10^3 CFU / ml. The coincidence rate of detection results by the developed method and that by plate agglutination test was 100%. Conclusion A specific and sensitive double antibody sandwich ELISA method for B. melitensis was developed.

关 键 词：羊布鲁菌 O链 M抗原 双抗体夹心ELISA 

分 类 号：S826[农业科学—畜牧学] S855.1[农业科学—畜牧兽医]