外周血SARP2基因甲基化检测对胰腺癌的诊断价值  

Diagnostic value of measurement of SARP2 methylation in peripheral blood for detection of pancreatic cancer

作　　者：宋健[1,2] 杜奕奇[1] 吕顺莉[1] 曹佳[1] 高军[1] 李兆申[1] 龚燕芳[1] 满晓华[1] 

机构地区：[1]第二军医大学附属长海医院消化科,上海200433 [2]解放军第401医院消化科

出　　处：《中华胰腺病杂志》2009年第4期229-231,共3页Chinese Journal of Pancreatology

基　　金：国家科技支撑计划课题（2006BA102A12）

摘　　要：目的探讨胰腺癌患者外周血DNA中SARP2基因甲基化对胰腺癌的诊断价值。方法收集12例原发性胰腺癌、10例慢性胰腺炎和6例健康志愿者外周静脉血，提取血清游离DNA，经亚硫酸氢盐修饰后，行SARP2基因第一外显子区域的BSP扩增和测序。结果12例胰腺癌外周血DNA、10例慢性胰腺炎外周血DNA中分别有10例（83％）和4例（40％）检测出明显的SARP2基因甲基化改变。胰腺癌患者外周血中SARP2基因第一外显子区CpG位点甲基化率为16．8％；慢性胰腺炎患者的甲基化率为10．4％，健康志愿者为2．2％。3组间的SARP2CpG岛甲基化率差别非常显著（P〈0．01或P〈0．05）。结论胰腺癌患者外周血DNA中可以检测到SARP2甲基化改变，SARP2甲基化的检测对胰腺癌的诊断可能有潜在的临床价值。Objective To investigate the diagnostic value of measurement of SARP2 methylation in peripheral blood for detection of pancreatic cancer in human. Methods Peripheral vein blood of 12 patients with primary pancreatic cancers, 10 patients with chronic pancreatitis and 6 health volunteers were collected. Serum free DNA were extracted from blood samples, and were modified with bisulfate, and SARP2 gene extron 1 were amplified through BSP and sequencing of the production. Results There were 12 patients （83%） with pancreatic cancer and 10 patients （40%） with chronic pancreatitis had obvious methylation in SARP2 gene in peripheral blood. The rate of CpG methylation in SAR[r2 gene extron 1 of pancreatic cancer, chronic pancreatitis and health volunteers was 16. 8% , 10. 4% and 2. 2% respectively. There was statistically significant difference among the three groups （P 〈 0.01 or P 〈 0.05 ）. Conclusions Aberrant methylation of SARP2 gene could be detected in peripheral blood in patients with pancreatic cancer, the detection of SARP2 gene methylation may have potential clinical implication for diagnosis of pancreatic cancer.

关 键 词：胰腺肿瘤 DNA甲基化 SARP2基因 

分 类 号：R735.9[医药卫生—肿瘤]