Clodronate脂质体对急性坏死性胰腺炎大鼠Kupffer细胞凋亡的作用  

Role of lipsomal clodronate on apoptosis of Kupffer cell in rat with acute necrotizing pancreatitis

作　　者：蔡华忠[1] 党胜春[1] 尹江涛[1] 崔磊[1] 陈敏[2] 姜德立[2] 张建新[1] 

机构地区：[1]江苏大学附属医院普外科,镇江212001 [2]江苏大学化学化工学院

出　　处：《中华胰腺病杂志》2009年第4期259-261,共3页Chinese Journal of Pancreatology

基　　金：国家自然科学基金资助项目（30772117）;江苏省自然科学基金资助项目（BK2007096）;镇江市社会发展基金项目（SH2008036）

摘　　要：目的探讨Clodronate脂质体对急性坏死性胰腺炎（acute necrotizing pancreatitis，ANP）大鼠Kupffer细胞（KC）凋亡的诱导作用。方法利用薄膜法制备Clodronate脂质体；采用胰腺被膜下均匀注射5％牛磺胆酸钠4ml／kg体重制作ANP模型，分离培养ANP大鼠KC，加入50、100、150μl的Clodronate脂质体进行干预，然后采用MTF法、流式细胞仪和DNA琼脂糖凝胶电泳技术检测KC细胞增殖、凋亡情况。结果所制备的脂质体直径100～200nm，透射电镜检查见其大小均匀；50、100、150μClodronate脂质体干预24h后，KC的生长抑制率分别为17．4％、24．2％和31．1％；细胞凋亡率分别为（14．12±0．37）％、（18．74±0．43）％和（27．51±0．39）％，差异均有显著性（P〈0．01）；随着Clodronate脂质体量的增加，KC的DNA出现降解，逐渐呈现清晰的特征性梯状条带。结论Clodronate脂质体对ANP大鼠KC生长具有明显抑制作用。并可诱导其凋亡。Objective To investigate the apoptosis of Kupffer cell （KC） induced by lipsomal clodronate in rat with acute necrotizing pancreatitis （ANP）. Methods Lipsomal clodronate was prepared by means of thin film, the model of ANP was established by injection of 5% sodium taurocholate of 4 ml/kg into the pancreatic capsule. The Kupffer cells were obtained from ANP rat. After exposure to different doses of lipsomal clodronate （0, 50, 100, 150 μl）, then the proliferation and apoptosis of KC was measured by MTT, flow cytometry and agarose gel electrophoresis of DNA. Results The prepared lipsomal clodronate had an average size of 100 - 200 nm, the spherical shape of liposome was uniform and confirmed by transmission electron microscope. When exposed to different concentration of lipsomal clodronate for 24 h, the growth suppression rate was 17.4%, 24.2% and 31. 1%, respectively, while the apoptosis rate of the KC was （ 14.12 ± 0.37 ） %, （ 18.74 ± 0.43 ） % and （ 27.51 ± 0.39 ） %, respectively ; the difference was statistically significantly （P 〈0.01 ）, the DNA of KC began degradation and gradually showed clear and characteristic ladder. Conclusions Lipsomal clodronate could induce apoptosis and suppress the growth of Kupffer cells in ANP rats.

关 键 词：胰腺炎 急性坏死性 枯否细胞 细胞凋亡 氯膦酸二钠 

分 类 号：R576[医药卫生—消化系统]