急性胰腺炎胰腺腺泡细胞胀亡和巨噬细胞激活的关系  

Relationship between the oncosis of pancreatic acinar cells and activation of macrophage in rat model of acute pancreatitis

作　　者：路广海[1] 张伟辉[1] 张迎媚[2] 薛东波[1] 孙备[1] 

机构地区：[1]哈尔滨医科大学附属第一医院普通外科,150001 [2]哈尔滨医科大学附属第一医院中心实验室,150001

出　　处：《中华消化外科杂志》2009年第4期275-277,共3页Chinese Journal of Digestive Surgery

基　　金：黑龙江省十一五科技攻关计划（GB07C32505）;黑龙江省卫生厅科研基金（2006-073）;黑龙江省教育厅科学技术研究项目（11531157）

摘　　要：目的探讨急性胰腺炎（AP）时胰腺腺泡细胞胀亡和巨噬细胞激活的关系。方法以两步酶消化法分离大鼠胰腺腺泡细胞，分为3组。AP组以蛙皮素制成AP细胞模型，实验组以蛙皮素＋内皮素处理，对照组加等量培养液。收集腺泡细胞以吖啶橙＋溴乙锭荧光染色检测细胞胀亡的情况。收集培养液部分上清液进行淀粉酶及LDH释放的检测；另取1ml上清液加入接种分离的大鼠腹腔巨噬细胞的培养板中培养6h，然后以ELISA法检测TNF-α蛋白水平。结果对照组细胞发生胀亡极少，胰腺腺泡细胞释放胰淀粉酶、LDH和巨噬细胞释放TNF-α也处于较低水平，分别为（1175±165）kU／L、（846±118）U／L、（36±5）μg／L；AP组可见细胞胀亡，胰淀粉酶、LDH的释放及TNF—α的水平均明显升高，分别为（7130±680）kU／L、（4262±626）U／L、（155±18）μg／L，与对照组比较差异有统计学意义（t=5．184，4．277，3．665，P〈0．05）；实验组各指标水平增高更明显，分别为（9240±1177）kU／L、（6937±893）U／L、（268±35）μg／L，与AP组比较差异有统计学意义（t=2．251，2．825，2．843，P〈0．05）。结论随着细胞胀亡的发生，胰腺腺泡细胞淀粉酶的释放增加，巨噬细胞释放TNF-α的变化趋势与细胞胀亡程度一致。巨噬细胞的炎症反应、胰腺腺泡细胞内容物的释放与胰腺腺泡细胞胀亡之间存在着联系。Objective To study the relationship between the oncosis of pancreatic acinar cells and activation of macrophage in rat model of acute pancreatitis （AP）. Methods The pancreatic acinar cells were isolated by two-step enzyme digestion, and then they were divided into control group, AP group and test group. Pancreatic acinar cells were cultured with caerulein in AP group, with caerulein and endothelin in test group, and with culture medium in control group. The oncosis rate of the pancreatic aeinar cells was detected after acridine orange and ethidium bromide fluorescent staining. The supernatant was collected to detect the release of amylase and lactate dehydrogenase （LDH）. The macrophages were cuhured with 1 ml of supematant for 6 hours, and then the protein level of tumor necrosis factor-α （ TNF-α） was measured by ELISA. Results Few oncotic pancreatic acinar cells were observed in the control group, and the levels of amylase and LDH secreted by pancreatic acinar cells and TNF-α secreted by maerophage were （ 1175 ± 165 ） kU/L, （ 846 ± 118 ） U/L and （ 36 ± 5 ）μg/L, respectively. Oncotic pancreatic acinar cells were observed in AP group, and the levels of amylase, LDH and TNF-α were （ 7130 ± 680） kU/L, （ 4262 ± 626 ） U/L and （ 155 ± 18 ）μg/L, respectively, which were significantly higher than those in control group （t =5. 184, 4. 277, 3. 665, P 〈0.05）. The levels of amylase, LDH and TNF-α were even higher in test group, and they were （9240 ± 1177）kU/L, （6937 ± 893）U/L and （268 ± 35 ）μg/L, respectively, which were significantly higher than those in AP group （ t = 2. 251, 2. 825, 2. 843, P 〈 0.05 ）. Conclusions The release of amylase was changed as the oncosis of pancreatic acinar cells occurred. The secretion of TNF-α was along with the degree of oncosis of pancreatic acinar cells. The results of the study indicate that a relationship exists among the inflammatory response of macrophage, the release of contents of pancreatic acinar cells and

关 键 词：急性胰腺炎 细胞胀亡 巨噬细胞 细胞因子 

分 类 号：R576[医药卫生—消化系统] R977.5[医药卫生—内科学]