咖啡酸苯乙酯干扰β-连环蛋白通路对大肠癌的影响  

Effects of caffeic acid phenethyl ester on the colorectal cancer via the β-catenin pathway

作　　者：何渝军[1] 刘宝华[1] 向德兵[2] 张菊馨[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所普通外科,重庆400042 [2]第三军医大学大坪医院野战外科研究所病理科,重庆400042

出　　处：《中华消化外科杂志》2009年第4期294-297,共4页Chinese Journal of Digestive Surgery

基　　金：重庆市科委自然基金（2006BB5054）

摘　　要：目的观察咖啡酸苯乙酯（CAPE）对大肠癌SW480细胞β-连环蛋白相关通路中β-连环蛋白、c—myc和细胞周期蛋白D1基因表达的影响。方法采用RT—PCR和Western blot方法检测不同浓度CAPE作用24、48h后，细胞β-连环蛋白、c-myc及细胞周期蛋白D1 mRNA和蛋白表达的变化。结果经不同浓度的CAPE作用后，SW480细胞β-连环蛋白、c-myc、细胞周期蛋白D1 mRNA和蛋白表达均有不同程度的下降，分别从1．05±0．26下降到0．67±0．10、0．87±0．09下降到0．51±0．14、0．63±0．09下降到0．32±0．14和204±52下降到52±16、111±11下降到52±16、87±7下降到32±12，与对照组（CAPE浓度为0mg／L）比较差异有统计学意义（F=5．724，6．793，7．026，15．936，14．889，14．162，31．147，28．881，6．322，17．647，9．584，P〈0．05），并呈剂量和时间依赖性。结论CAPE可干扰β-连环蛋白相关通路，下调β-连环蛋白及通路靶基因c-myc和细胞周期蛋白D1的转录和表达。CAPE抗肿瘤作用可能与下调β-连环蛋白通路相关基因表达有关。Objective To study the effects of caffeic acid phenethyl ester （CAPE） on the expression of β-eatenin, c-myc and cyclin D1 in eoloreetal cancer cell line SW480. Methods The changes of mRNA and protein expression of β-catenin, cyclin D1 and c-myc were detected by RT-PCR and Western blot after culturing the colorectal cancer cell line SW400 with different concentrations of CAPE （2.5,5.0, 10.0 mag/L） for 24 hours and 48 hours. Results After the treatment of CAPE, the mRNA expression of β-catenin,cyclin D1 and c-myc were decreased from 1.05 ±0.26, 0.87 ±0.09, 0.63 ±0.09 to 0.67 ±0. 10, 0.51 ±0. 14, 0.32 ±0. 14, respectively, and the protein expression of β-catenin, eye/in D1 and c-myc were decreased from 204 ± 52, 111 ± 11, 87 ± 7 to 52 ±16, 52 ± 16, 32 ± 12, respectively. There was a significant difference in the decrease of mRNA and protein expression of β-catenin, cyclin D1 and c-myc in colorectal cancer cell line SW480 with and without treatment of CAPE （F=5. 724, 6. 793, 7. 026, 15. 936, 14. 889, 14. 162, 31. 147, 28. 881, 6. 322, 17＋ 647, 9.584, P 〈0.05） The inhibition effect of CAPE was displayed in a dose- and time-dependent manner. Conclusions CAPE can obstruct the β-catenin pathway, and down-regulate the transcription and expression of β-catenin, cyclin D1 and c-myc. The anti-tumor effect of CAPE may be related to the decreased expression of β-catenin, cyclin D1 and c-myc.

关 键 词：大肠肿瘤 咖啡酸苯乙酯 Β-连环蛋白 

分 类 号：R735.34[医药卫生—肿瘤]