中线T细胞淋巴瘤的TCR-γ基因重排研究  

Detection of T cell receptor γ Chain gene rearrangement in midline T cell lymphomas by PCR method

作　　者：陈德忠[1] 刘卫平[1] 刘永惠 李甘地[1] 杨光华 李俸媛 

机构地区：[1]华西医科大学第一附属医院病理科

出　　处：《中华病理学杂志》1998年第3期198-201,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金;美国中华医学基金

摘　　要：目的对中线Ｔ细胞淋巴瘤的克隆性进行研究。方法用一组针对Ｔ细胞受体γ链基因Ｖ区片段的家族特异性引物和ＰＣＲ方法，对１１例（２２个标本）中线Ｔ细胞淋巴瘤病例进行了Ｔ细胞受体γ基因重排的检测。结果２２个标本中２１个有Ｔ细胞受体γ链基因的克隆性重排（９４４５％）。在９例原发灶的连续活检和１例原发灶及其转移灶的标本中均未发现增生的克隆家族的改变。结论中线Ｔ细胞淋巴瘤的病变组织中存在Ｔ细胞的单克隆性增生，为该肿瘤的Ｔ细胞起源提供了分子生物学的证据。在中线Ｔ细胞淋巴瘤的疾病过程中（包括转移灶）增生Ｔ细胞的家族未发生变化。Objective To investigate the clonality of midline T cell lymphoma. Methods Using polymerase chain reaction method and a panel of family specific primers for the variable regions of TCR γgene, and totally 11 cases (22 samples) of midline T cell lymphoma (midline malignant reticulosis) were studied. Results Family specific monoclonal TCR γgene rearrangements were found in 21 out of 22 samples (94.45%). There was no change of clonality during the course of disease. Conclusion There is monoclonal T cell proliferation in midline T cell lymphoma and the clonality is not transformed with the advance of the disease. This finding provides an evidence to support the hypothesis of monoclonal origin of neoplasm.

关 键 词：淋巴瘤 T细胞 基因重排 

分 类 号：R733.402[医药卫生—肿瘤]