亚硒酸钠对人早幼粒白血病细胞的RB蛋白磷酸化的影响  

Effect of sodium selenite on phosphorylation of retinoblastoma protein of human promyelocytic leukemia cells (HL-60)

作　　者：刘新光[1] 于树玉[2] 

机构地区：[1]广东医学院医用生化研究所,湛江524023 [2]中国医学科学院肿瘤研究所生化室

出　　处：《肿瘤防治研究》1998年第4期249-251,共3页Cancer Research on Prevention and Treatment

摘　　要：以人早幼粒白血病细胞（HL－60）为实验对象，利用Western－blotting方法和流式细胞仪分析，研究亚硒酸钠对HL－60细胞的RB蛋白产物磷酸化的影响和探讨其抗癌机制。Western－blotting结果显示：与对照组相比，在第四天5．8μmol／LNa2SeO3可使磷酸化Rll蛋白减少49％，去磷酸化RB蛋白仅轻度增加，但在加硒组中去磷酸化RB蛋白占RB蛋白总量的71．1％。与此同时，细胞周期的结果表明：亚硒酸钠对HL－60细胞的G2＋M和S期有一定的阻滞。该实验在蛋白分子水平上研究了亚硒酸钠抑制HL－60细胞增殖的作用机制。Human promyelocytic leukemia cells (HL-60) was used to study the effect of sodiumselenite on phosphorylation of retinoblastoma (RB) protein and its anticancer mecha-nism. Method of western--blotting and flow cytometric analysis were undertaken. Theresult of western-blotting showed that 4 days after treatment of 5. 8μmol/L. Na2SeO3,the amounts of the phosphorylated RB protein decreased some 49%that of controlgroup,while the hypophosphorylated RB protein increased a little. However, in this selenium group the phosphorylated RB protein was 71.1 % in total RB protein amounts.In the same time, the result of cell cycle revealed that sodium selenite could slightly arrest HL-60 cells at G2+M and S phase. The experiments studied the mechanism of sodium selenite inhibited HL-60 cells proliferation at protein molecules level.

关 键 词：白血病 HL-60细胞 亚硒酸钠 RB蛋白磷酸化 

分 类 号：R733.705[医药卫生—肿瘤] R73－36[医药卫生—临床医学]