RB、P^(53)抑癌基因蛋白在甲状腺肿瘤中的表达  

Expression of retinoblastoma and P^(53) tumour suppressor gene protein in human thyroid tumours

作　　者：蒋昌林[1] 丁祖玖[1] 黄裕华 汪谟庆 石■磊 

机构地区：[1]安徽医科大学病生教研室,合肥230032 [2]安徽巢湖地区医院病理科 [3]安徽芜湖市一院病理科 [4]解放军105医院病理科

出　　处：《肿瘤防治研究》1998年第4期270-272,共3页Cancer Research on Prevention and Treatment

摘　　要：ABC免疫组化法检测77例甲状腺肿瘤和非瘤病变中RB与P53抑癌基因蛋白的表达。所有标本中都有RB蛋白表达，表明RB基因失活在甲状腺肿瘤中不起重要作用。甲状腺非瘤病变与腺瘤中P53全部阴性，甲状腺乳头状癌、滤泡状癌中P53阳性率低（8．3％与15．8％），阳性细胞数也少（<10％），P53主要在甲状腺未分化癌中高表达（66．7％）且与乳头状癌，滤泡状癌有显著性差异，提示P53蛋白异常在甲状腺癌由高分化向未分化发展过程中起关键性作用。We have examined retinoblastoma (RB) and p53protein by immunohistochemistry inseventy-seven formalin fixed, paraffin wax embedded thyroid lessions. Thyroid lessionsstudied included 10 non-neoplastic thyroid tissues, 14 thyroid adenomas, 24 papillarycarcinomas, 19 follicular carcinomas, 9 undifferentiated carcinomas and 1 medullary car-cinoma. The RB protein is not loss in any cases, indicating that inactivatlon of the RBgene is unlikely to play a important role in the pathogenesis of thyroid tumours. Six of 9(66. 7%) undifferentiated carcinonlas, 3 of 19(15. 8%) follicular carcinomas, 2 of 24 (8.3% ) papillary carcinomas show p53protein nuclear staining. In all of p53positive cases, 4of 6 undifferentiated carclnomas showed more positive cells than 5 well-differentiatedcarcinomas in which less than 10 percent of cells had increased p53protein levels. Our results suggest that p53protein abnormalities play a crucial role in the progression of well-differentiated to undifferentiated thyroid carcinomas.

关 键 词：P^53蛋白 RB蛋白 甲状腺肿瘤 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]