芦笋多糖对S180小鼠红细胞补体受体-1(CR1,CD35)数量及活性的影响  

Effect of Asparagus Polysaccharide on the Number and Activity of Erythrocyte Complement Receptor 1(CD35) of S180 Mice

作　　者：季宇彬[1,2] 闵永萃[1,2] 汲晨锋[3] 

机构地区：[1]哈尔滨商业大学国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076 [2]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [3]哈尔滨商业大学药物研究所博士后科研工作站,哈尔滨150076

出　　处：《中国药学杂志》2009年第14期1066-1069,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30400592);哈尔滨市青年基金资助项目(P260013)

摘　　要：目的研究探讨芦笋多糖对S180小鼠红细胞补体受体-1(CR1)数量、红细胞CR1天然免疫活性的影响。方法取小鼠的新鲜红细胞,用大鼠抗小鼠CD35一抗和FITC-羊抗大鼠二抗标记小鼠红细胞,应用流式细胞仪测定小鼠红细胞CR1数量;用红细胞免疫花环实验检测红细胞C3b受体花环率(RBC-C3bRR)和肿瘤红细胞细胞花环率(DTER)。结果芦笋多糖高剂量组和中剂量组使S180小鼠红细胞CR1数量和RBC-C3bRR、DTER显著性增加。结论芦笋多糖可以改善S180小鼠红细胞免疫功能,可能是其抗肿瘤作用的机制之一。OBJECTIVE To study the effect of Asparagus o3ficinalis polysaccharide on the number and activity oferythroeyte complement receptor-1 in S180 mice. METHODS Red blood cells from mice venous blood were labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, the number of ECR1 was determined. Using microscope, the adherence between erythrocyte immunity and C3b receptor or tumor-cell was investigated by RBC-C3bRR and DTER. RESULTS Comparing with control groups, the mean value of the number of CR1, RBC-C3bRR and DTER of Asparagus officinalis polysaccharide groups were increased significantly. CONCLUSION Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice, which may be one of its most important antitumor mechanisms.

关 键 词：芦笋多糖 红细胞 C3B受体花环率 

分 类 号：R284[医药卫生—中药学]