中华眼镜蛇毒对荷人鼻咽癌裸鼠血浆MDA和SOD的影响  

Effects of naja naja atra venom on plasmic MDA and SOD of human NPC-bearing nude mice

作　　者：杨惠玲[1,2] 马志楷[1,2] 余清声 郑芹[1,2] 

机构地区：[1]中山医科大学病理生理学教研室 [2]广州医学院蛇毒研究所

出　　处：《癌症》1998年第4期249-250,253,共3页Chinese Journal of Cancer

基　　金：广东省自然科学基金

摘　　要：目的：为了探讨中华眼镜蛇毒（ＮＮＡＶ）对荷人鼻咽癌（ＮＰＣ）裸鼠血浆丙二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）活性的影响。方法：采用比色法检测经腹腔注射低、中或高浓度ＮＮＡＶ（１μｇ／ｍｌ、５μｇ／ｍｌ、１０μｇ／ｍｌ）的荷人ＮＰＣ裸鼠血浆ＭＤＡ和ＳＯＤ活性。结果：荷瘤鼠血浆ＭＤＡ（８４２ｎｍｏｌ／ｍｌ）比正常裸鼠（３９２ｎｍｏｌ／ｍｌ）明显升高，而荷瘤鼠血浆总ＳＯＤ（Ｔ－ＳＯＤ）、锰ＳＯＤ（Ｍｎ－ＳＯＤ）和铜锌ＳＯＤ（ＣｕＺｎ－ＳＯＤ）活性（５２１９Ｎｕ／ｍｌ，２０３１Ｎｕ／ｍｌ，３１８８Ｎｕ／ｍｌ）比正常裸鼠（１２５６４Ｎｕ／ｍｌ，７５．１１Ｎｕ／ｍｌ，５０．５３Ｎｕ／ｍｌ）低；低、中和高浓度ＮＮＡＶ均可使荷瘤鼠血浆ＭＤＡ降低或至正常范围（５６２ｎｍｏｌ／ｍｌ，５．１７ｎｍｏｌ／ｍｌ，４．５８ｎｍｏｌ／ｍｌ），并可使荷瘤鼠Ｔ－ＳＯＤ、Ｍｎ－ＳＯＤ和ＣｕＺｎ－ＳＯＤ有不同程度的升高，其中以高浓度组为佳（１０８８８Ｎｕ／ｍｌ，７２２７Ｎｕ／ｍｌ，３６６１Ｎｕ／ｍｌ，）。结论：结果提示ＮＮＡＶ的抑瘤作用可能与其降低荷瘤鼠自由基水平和提高ＳＯＤ活性有关。Objective:To investigate the changes of plasma malondialdehyde(MDA)and superoxide dismutase(SOD)activitg in nude mice(NM)bearing human nasopharyngeal carcinoma(NPC)and observe the effects of Naja Naja atra(NNAV) on them.Methods:Plasma MDA and SOD in human NPC-bearing NM treated by low、 middle or hight-concentration NNAV solution (l μg/ml,5 μg/ml,10 μg/ml)peritoneally were determined by colorimetry.Results:Plasma MDA content(8.42 nmol/ml) in NM bearing tumor increased markedly in comparison with the control group (3.92 nmol/ml).Whereas plasma T-SOD、Mn-SOD and Cu-Zn-SOD activity (52.19 Nu/ml,20.31Nu/ml,31.88 Nu/ml) in NM bearing tumor control group(125.64 Nu/ml,75.11 Nu/ml,50.53 Nu/ml) decrease significantly in comprison with blank control group. Low,middle or high-concentration NNAV solution(especial hight-concentration group) decreased plasma MDA content and enhanced SOD activity in NM bearing tumor. Conclusion:The antitumor effect of NNAV might be by means of decreasing free redicals and increasing SOD activity in NM bearing tumor.　　

关 键 词：中华眼镜蛇毒 鼻咽癌 丙二醛 超氧化物歧化酶 

分 类 号：R739.63[医药卫生—肿瘤] R73－36[医药卫生—临床医学]