宫颈癌变过程中14-3-3σ基因mRNA的表达  

Expressions of 14-3-3σ in cervical cancer and cervical intraepithelial neoplasia

作　　者：肖巍[1,2] 卞美璐[2] 徐波[3] 

机构地区：[1]北京协和医学院研究生院,北京100730 [2]中日友好医院妇产科,北京100029 [3]中日友好医院临床医学研究所,北京100029

出　　处：《中日友好医院学报》2009年第3期163-165,共3页Journal of China-Japan Friendship Hospital

摘　　要：目的:通过检测宫颈病变组织中14-3-3σ基因的mRNA含量,探讨其在宫颈癌发生发展过程中的表达与意义。方法:应用实时荧光定量聚合酶链反应(RFQ-PCR)定量检测14-3-3σmRNA在正常宫颈、宫颈上皮内瘤变及宫颈癌组织中的表达。结果:RFQ-PCR结果表明宫颈癌组的14-3-3σmRNA水平显著高于正常宫颈组,而宫颈上皮内瘤变组14-3-3σmRNA水平与正常宫颈相近。结论:在宫颈癌中,14-3-3σ转录上调,而宫颈上皮内瘤变中14-3-3σ转录没有明显变化,14-3-3σ在宫颈癌变过程中可能发挥作用。Objective：To explore the expressions of 14-3-3σ in cervical cancer and cervical intraepithelial neoplasia（CIN）.Methods：The mRNA expression of 14-3-3σ was evaluated by means of real-time fluorogenetic quantitative polymerase chain reaction （RFQ-PCR） in cervical tissues of 28 cervicitis,30 CIN1,30 CIN2,30 CIN3 and 16 cervical cancers.Results：RFQ-PCR showed that the average quantity of 14-3-3σ mRNA in cervical cancer tissues was significantly higher than that in cervicitis tissues（P〈0.05）.While the average quantities of 14-3-3σ mRNA in CINs were similar with cervicitis tissues.Conclusion：14-3-3σ is overexpressed in cervical cancer tissues on transeriptional level,but not in CINs tissues.So 14-3-3σ may play an important role in the pathogenesis of cervical cancer.

关 键 词：宫颈癌 14-3-3σ 实时荧光定量聚合酶链反应 

分 类 号：R737.33[医药卫生—肿瘤]