天花粉蛋白的二级结构和B细胞抗原表位预测  

Prediction of the Secondary Structure and B-cell Antigen Epitope on Trichosanthin

作　　者：王媛[1] 许晶[2] 黄艳平[1] 邢爱华[2] 常文辉[2] 贾华[2] 王平忠[3] 白文涛[1] 徐志凯[1] 黎志东[1] 

机构地区：[1]第四军医大学基础部微生物学教研室,西安710032 [2]陕西省疾病预防控制中心,西安710054 [3]第四军医大学唐都医院全军感染病诊疗中心,西安710038

出　　处：《科学技术与工程》2009年第17期4915-4917,4929,共4页Science Technology and Engineering

基　　金：国家自然科学基金(30671877);陕西省科学技术研究发展计划(2006K11-G3)资助

摘　　要：为预测天花粉蛋白(TCS)的B细胞抗原表位,采用生物信息软件和互联网服务器,预测TCS的二级结构和分析TCS表面特性(亲水性、可塑性、可及性和抗原性);再计算平均抗原指数(AI),预测TCS的B细胞抗原表位。结果TCS存在多个潜在的抗原表位,24～32序列(LPNERKLY)的AI(0.394)明显高于其他片段,24～32序列是TCS潜在的B细胞优势抗原表位。说明TCS的定点突变及免疫原性的降低提供了理论指导。To predict the B-cell epitope for Trichosanthin antigen, using biotic software and network servers, the secondary structure of TCS was predicted, and the surface properties of TCS, such as hydrophilicity, flexibility, accessibility and antigenicity,were analyzed. Then the average antigen index （AI） was calculated by using Wu＇s method. It is result ed that many distinct antigenic epitopes on TCS were identified： AI of sequence 24 - 32 （LPNERKLY） on TCS was 0. 394, significantly higher than that of other sequences. Sequence 24 - 32 was the prevalent antigen epitope of B-cell on TCS. conclused that B-ceU epitopes of TCS is predicted successfully.

关 键 词：抗原表位 人类免疫缺陷病毒 生物信息学 

分 类 号：R373.9[医药卫生—病原生物学]