栓线法制备大鼠局灶性脑缺血再灌注模型的研究  

A Rats Model of Local Cerebral IschemiaReperfusion by Cylinder Embolization

作　　者：何秋[1,2,3] 刘美洁 朴英善[1,2,3] 魏秀云 王慕一 

机构地区：[1]辽宁省人民医院公费医疗科 [2]哈尔滨市红十字中心医院神经内科 [3]辽宁省人民医院检验科

出　　处：《中国医科大学学报》1998年第4期343-346,共4页Journal of China Medical University

摘　　要：目的：建立一种可靠、实用的局部脑缺血再灌注模型。方法：用尼龙线栓塞大脑中动脉（ＭＣＡ）制做局部脑缺血再灌注模型。观察大鼠脑缺血不同时间再灌注的成功率、神经经病学评分、丙二醛（ＭＤＡ）含量、脑水肿及病理学变化。结果：ＭＣＡ闭塞２４ｈ后，大鼠出现脑水肿、脂质过氧化和不同程度的神经损伤症状；光镜下见额顶叶皮质及基底节区梗塞。早期再灌注可明显改善神经损伤症状，减轻脑水肿，而脂质过氧化及病理损害加重。改进实验方法后，大鼠脑缺血不同时间再灌注的成功率明显提高。Objective:This reserch was to build a reliable, practical model of focal cerebral ischemia and reperfusion in rats. Methods:We made a model of occlusion and reperfusion in the middle cerebral artery (MCA) of rats by introducing nylon suture into internal carotid artery in rats group 1, 2, and 3 underwent one, three, and 24 hours of ischemia respectively. The successful rate of reperfusion (SRR), neurologic deficit score, brain edema, pathological changes and MDA level are measured at 24 hours later than the onset of ischemia. Results: ①Group 3 represented the neurologic deficit (score:2.33±1.00) and the damages of frontoparietal cortex and basal ganglia. The brain edema, MDA level of group 3 increased significantly compared with the shamoperation group (P<0.001, P<0.05 respectively). ②Both the brain edema and the neurologic deficit score of group 1 and group 2 were lower than that of group 3 (P<0.001, P<0.05 respectively), but the MDA level of group 1 and group 2 was nigher that of group 3 (P<0.001, P<0.05 respectively), The cortex and basal ganglia were damaged most severely. ③ By the improved model, the SRR at 1, 3 hour after ischemia were 90.5%, 84.6%. Conclusions: It's a relatively ideal animal model of the focal cerebral ischemia and reperfusion.

关 键 词：脑缺血 再灌注模型 栓线法 大鼠 动物模型 

分 类 号：R743.31[医药卫生—神经病学与精神病学]