小干扰RNA抑制肝癌HepG2细胞VEGF表达的研究  

Study of Inhibition Effects of Small Interfering RNA on VEGF Gene Expression in HepG2 Hepatic Cancer Cell

作　　者：朱洪波[1] 陈兆永[1] 王勇[1] 周晓春[1] 王建新[2] 

机构地区：[1]江苏省连云港市第一人民医院东方医院普外科,连云港222042 [2]江苏省南通市第三人民医院普外科,南通226000

出　　处：《中国普外基础与临床杂志》2009年第8期633-636,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的观察小干扰RNA(small interference RNA,si RNA)对肝癌HepG2细胞血管内皮生长因子(VEGF)表达的抑制作用。方法将VEGFsi RNA经LipofectamineTM2000脂质体转染肝癌HepG2细胞后,实时荧光定量PCR和Western blot方法分别检测VEGF mRNA和蛋白的表达,ELISA检测细胞培养上清液中VEGF蛋白浓度。结果细胞培养6 h后si RNA的转染效率为(90.4±2.9)%,转染后肝癌HepG2细胞中VEGF mRNA和蛋白的表达明显减少,细胞培养上清液中VEGF蛋白表达下降。结论运用RNA干扰技术,可以有效地干扰肝癌HepG2细胞VEGF的表达并能降低VEGF的生成。Objective To observe the effect of RNA interference （RNAi） on HepG2 hepatic cancer cell by small interfering RNA （siRNA）. Methods siRNA targeting vascular endothelial growth factor （VEGF） gene was transfected into HepG2 cells by LipofectimineTM 2000. The VEGF mRNA and protein were respectively detected by real-time quantitive PCR and Western blot, and the concentration of VEGF protein in the cell culture supertant was determined by ELISA at 48 h after culture. Results The average efficiency of siRNA transfection was （90. 4 ± 2.9） % after 6 h cell culture. The expressions of VEGF mRNA and protein in HepG2 cells could be effectively suppressed by siRNA, and the concentration of VEGF protein in the cell culture supertant was also decreased. Conclusion siRNA can knock down the expression of VEGF gene and decrease the concentration of VEGF protein in HepG2 cells.

关 键 词：RNA干扰 小干扰RNA 肝癌细胞 血管内皮生长因子 基因工程 基因治疗 

分 类 号：R735.7[医药卫生—肿瘤]