喉鳞状细胞癌生物学行为与VEGF-C和VEGFR-3的关系  

Relationships of Human Laryngeal Squamous Cell Carcinomas with the Expression of VEGF-C and VEGFR-3

作　　者：王中亮[1] 李瑞祥[2] 周斌[3] 梁伟波[3] 梁传余[4] 张林[3] 陈尧[2] 

机构地区：[1]江苏省苏北人民医院耳鼻咽喉科,扬州225002 [2]四川大学华西基础医学与法医学院人体解剖教研室,成都610041 [3]四川大学华西基础医学与法医学院法医物证教研室,成都610041 [4]四川大学华西医院耳彝咽喉科,成都610041

出　　处：《生物医学工程学杂志》2009年第4期842-846,共5页Journal of Biomedical Engineering

摘　　要：为了探讨喉鳞状细胞癌的生物学行为与血管内皮生长因子C(VEGF-C)和血管内皮生长因子受体3(VEGFR-3)之间的关系,我们采用逆转录聚合酶链式反应技术(RT-PCR),从基因水平上对30例正常喉黏膜组织、喉鳞癌组织及颈淋巴结组织中的VEGF-C、VEGFR-3表达进行检测;并采用蛋白质印迹(western bloting)方法从蛋白水平上对10例正常喉黏膜组织、喉鳞癌组织及颈淋巴结组织中的VEGF-C、VEGFR-3表达进行检测。其结果表现为:对同一患者,VEGF-C、VEGFR-3在喉鳞癌组织、颈淋巴结组织及正常喉黏膜组织中的表达有显著性意义(P<0.05),在喉鳞癌组织中,VEGF-C、VEGFR-3的表达与淋巴结转移以及病理分级有关(P<0.05),而与患者年龄性别、病变部位、T分期无关(P>0.05)。由此我们认为喉鳞状细胞癌生物学行为与VEGF-C和VEGFR-3之间有密切的关系。VEGF-C、VEGFR-3联合检测可望作为判断喉鳞癌患者预后的指标。In order to detect the relationships of human laryngeal squamous cell carcinoma （LSCC） with the expression of Vascular Endothelial Growth Factor C （VEGF-C） and Vascular Endothelial Growth Factor Receptor-3 （VEGFR-3）, we examined the level of VEGF-C and VEGFR-3 gene expression in the samples from 30 cases of normal laryngeal mucosa tissue（NLM）, primary laryngeal carcinoma （PLC） and cervical lymph node （CLN） by using reverse transcription polymerase chain reaction （RT-PCR）. The protein levels of VEGF-C and VEGFR-3 expression were also determined by Western blotting in 10 cases of NLM, PLC, CLN,respectively. We found that in the samples from the same patient, the VEGF-C and VEGFR-3 expression level is different among normal laryngeal mueosa tissue, primary laryngeal carcinoma and cervical lymph node. In primary laryngeal carcinoma, the level of VEGF-C and VEGFR-3 expression was significantly higher in lymph node positive group than in lymph node negative group, and this difference was the associated with the histological grade of differentiation（P〈0. 05）. The expression levels of VEGF-C and VEGFR-3 were not associated with age, sex, site and T stage（P〉0.05）. These results suggest that there is a close relationship of LSCC with VEGF-C/VEGFR-3 ; and VEGF-C / VEGFR-3 may have the potential for use as an indicator with prognostic significance in dealing with LSCC.

关 键 词：喉鳞状细胞癌 逆转录聚合酶链式反应技术 蛋白质印迹 血管内皮生长因子C 血管内皮生长因子受体3 

分 类 号：R739.6[医药卫生—肿瘤]